别名： NM_001034943.1；Rst; Slc22al2; URAT1
原核抗性： Amp
筛选标记： Puro
克隆菌株： Stbl3
培养条件： LB/37℃
序列：
CDS complement(8496..9356)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9357..9461)
/gene="bla"
/note="AmpR promoter"
polyA_signal 9509..9643
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac
2821 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
2881 aagctcgaga tggcgtttcc tgaactcctg gaccgagtag ggggccgggg caggttccag
2941 ctcctccagg cagtggctct ggtgaccccc attttgtggg tcaccaccca gaacatgctg
3001 gagaacttct cagccgcagt tccccaccac cgctgttggg tgcctcttct ggacaacagc
3061 acctcccagg cgagtatccc tggggacttt ggacgagacg ttcttctggc cgtctccatc
3121 ccacctggtc cggatcagcg accccaccag tgcctccgct tccgacaacc tcagtggcag
3181 cttatagagt ccaacaccac ggccaccaac tggagcgacg ctgacaccga gccatgtgag
3241 gatggctggg tttacgacca cagtaccttc aggtccacaa ttgtgaccac gtgggacctg
3301 gtatgtgatt cccaggctct gagacccatg gcccagtcca tcttcctggc tggaatcctg
3361 gtaggagccg cagtgtgcgg ccatgcctca gataggtttg ggcgcagaag ggtgctgacc
3421 tggagctatc ttctggtgtc tgtgtctggc acaatagctg ccctcatgcc caccttcccc
3481 ctctactgcc tgttccgttt cctggtggcc tctgcagtgg caggtgtcat gatgaacaca
3541 gccagcctct tgatggagtg gacatcagcc caggctggcc ccttgatgat gaccttgaac
3601 gccttgggct tcagctttgg gcaggtcctg acaggctctg tggcctacgg cgtgcgcagc
3661 tggaggatgc tgcagttggc tgtctctgcc cccttcttcc tcttctttgt ttactcatgg
3721 tggctgccag aatcagcacg ctggctcatc acagtgggca gactggacca gagcctgcgg
3781 gaactacaga gggtggctgc tgtcaacagg agaaaggcag aggcagacac gttgactgtg
3841 gaggtcctgc ggtcagccat gcaggaggaa ccgaacggga accaagctgg tgcccgtttg
3901 ggcactctac tccacacacc tgggctgcgc ctccgaacct tcatctccat gctgtgctgg
3961 tttgcctttg gcttcacctt ctatggcctg gcccttgacc tgcaagccct aggaagcaat
4021 atcttcctgc tccaggcact catcgggatt gtggacctcc cggtgaagat gggcagcctg
4081 ctgctgctca gccgcttggg ccggcgcctc tgccaggcca gctccctggt gctgccggga
4141 ctctgcatcc tggccaacat actggtgccc cgtgagatgg ggatccttcg ctcatccttg
4201 gctgtgctgg ggctggggtc cctgggggct gccttcacct gtgtcaccat attcagcagt
4261 gaactcttcc ccactgtgat caggatgact gcagtgggcc tgggccaggt agcagcccga
4321 ggaggagcca tgctagggcc tttggtgcgg ctgctgggtg tctatggctc ctggctgcct
4381 ctgctggtgt atggagtggt gccagtgtta agcggcctag ctgcattgct gctgcctgag
4441 accaagaact tgccactgcc tgacaccatc caagacatcc agaaacagtc agtgaagaaa
4501 gtgacacatg acatagcagg cggctccgtc ctgaagtctg cccggttgta aggatcccgc
4561 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
4621 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
4681 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
4741 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
4801 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
4861 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
4921 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
4981 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
5041 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
5101 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac
5161 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
5221 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
5281 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
5341 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
5401 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
5461 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
5521 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
5581 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
5641 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
5701 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
5761 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
5821 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
5881 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5941 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
6001 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
6061 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
6121 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
6181 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
6241 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
6301 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
6361 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
6421 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
6481 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
6541 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
6601 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
6661 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
6721 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
6781 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
6841 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
6901 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
6961 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
7021 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
7081 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
7141 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
7201 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
7261 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
7321 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
7381 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
7441 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
7501 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
7561 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7621 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
7681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
7741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
7801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
7861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
7921 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
7981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
8041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
8101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
8161 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
8221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
8281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
8341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
8401 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
8461 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
8521 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
8581 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
8641 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
8701 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
8761 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
8821 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
8881 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
8941 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
9001 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
9061 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
9121 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
9181 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
9241 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
9301 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
9361 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
9421 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
9481 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
9541 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
9601 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
9661 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
9721 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
9781 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt
9841 actacaaact tagtagt
