序列：
CDS complement(10431..11225)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt
2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggtgagtc
4381 tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga
4441 gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
4501 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
4561 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
4621 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
4681 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
4741 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
4801 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
4861 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
4921 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
4981 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
5041 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
5101 ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt
5161 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
5221 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
5281 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
5341 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
5401 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
5461 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
5521 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
5581 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
5641 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
5701 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
5761 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
5821 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
5881 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
5941 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
6001 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
6061 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
6121 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
6181 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
6241 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
6301 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
6361 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
6421 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
6481 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
6541 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
6601 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
6661 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
6721 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
6781 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
6841 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
6901 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
6961 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
7021 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
7081 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
7141 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
7201 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
7261 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
7321 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
7381 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
7441 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
7501 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
7561 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
7621 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
7681 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
7741 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
7801 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
7861 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
7921 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
7981 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
8041 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
8101 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
8161 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
8221 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
8281 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
8341 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
8401 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
8461 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
8521 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
8581 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
8641 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
8701 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
8761 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
8821 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
8881 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
8941 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
9001 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
9061 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
9121 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
9181 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
9241 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
9301 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
9361 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
9421 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
9481 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
9541 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
10321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
10381 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
10441 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
10501 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
10561 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
10621 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
10681 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
10741 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
10801 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
10861 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
10921 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
10981 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
11041 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
11101 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
11161 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
11221 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
11281 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
11341 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
11401 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
11461 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
11521 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
11581 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
11641 gctggctgg
