终止子： OCS terminator
原核抗性： 卡那霉素Kan
筛选标记： 草铵膦Bar
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃
序列：
CDS complement(9164..9958)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
3061 aaagtggtgg aacagaaact gatctctgaa gaagatctgt gactaggtga gtctagagag
3121 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct
3181 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
3241 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
3301 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
3361 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
3421 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
3481 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
3541 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
3601 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
3661 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
3721 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
3781 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
3841 aacatgtcga ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta
3901 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
3961 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
4021 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
4081 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
4141 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
4201 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
4261 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
4321 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
4381 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
4441 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
4501 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
4561 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
4621 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
4681 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
4741 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
4801 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
4861 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
4921 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
4981 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
5041 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
5101 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
5161 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
5221 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
5281 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
5341 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
5401 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
5461 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
5521 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
5581 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
5641 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
5701 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
5761 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
5821 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
5881 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
5941 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
6001 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
6061 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
6121 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
6181 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
6241 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
6301 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
6361 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
6421 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
6481 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
6541 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
6601 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
6661 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
6721 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
6781 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
6841 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
6901 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
6961 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
7021 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
7081 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
7141 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
7201 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
7261 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
7321 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
7381 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
7441 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
7501 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
7561 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
7621 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
7681 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
7741 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
7801 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
7861 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
7921 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
7981 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
8041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
8101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
8161 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
8221 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
8281 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
8341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
8401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
8461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
8521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
8581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
8641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
8701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
8761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
8821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
8881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
8941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
9001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
9061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
9121 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
9181 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
9241 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
9301 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
9361 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
9421 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
9481 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
9541 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
9601 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
9661 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
9721 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
9781 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
9841 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
9901 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
9961 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
10021 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
10081 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
10141 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
10201 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
10261 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
10321 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
10381 gg
