培养条件：
37℃
二,质粒简介：
Human ORFeome Gateway entry vector pENTR223-PMS1
三,质粒图谱及序列：
rep_origin 2457..3045
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg aaacaattgc ctgcggcaac agttcgactc ctttcaagtt ctcagatcat
541 cacttcggtg gtcagtgttg taaaagagct tattgaaaac tccttggatg ctggtgccac
601 aagcgtagat gttaaactgg agaactatgg atttgataaa attgaggtgc gagataacgg
661 ggagggtatc aaggctgttg atgcacctgt aatggcaatg aagtactaca cctcaaaaat
721 aaatagtcat gaagatcttg aaaatttgac aacttacggt tttcgtggag aagccttggg
781 gtcaatttgt tgtatagctg aggttttaat tacaacaaga acggctgctg ataattttag
841 cacccagtat gttttagatg gcagtggcca catactttct cagaaacctt cacatcttgg
901 tcaaggtaag aaagtagctt tgtatacaaa cattctttac cttttctgtc ttaattgttg
961 gttaaaaaaa aaaaagttac tcggtgccca actttcttgt acaaagttgg cattataaga
1021 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
1081 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
1141 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
1201 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctgcccaag
1261 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
1321 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
1381 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
1441 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
1501 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
1561 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
1621 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
1681 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
1741 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
1801 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
1861 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
1921 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
1981 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
2041 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
2101 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
2161 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
2221 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
2281 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
2341 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
2401 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
2461 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
2521 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
2581 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
2641 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
2701 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
2761 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
2821 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
2881 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
2941 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
3001 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
3061 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
3121 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
3181 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
3241 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggca
图谱：
