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p53-ABAI质粒

货号：kl-zl-0561-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0561-01	p53-ABAI质粒	NA		立即咨询	+ -

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p53-ABAI质粒货号：kl-zl-0561 规格：20ul

启动子：	URA3
复制子：	pUC ori
质粒分类：	酵母系列，酵母单杂交载体
质粒大小：	4944bp
原核抗性：	Amp
筛选标记：	URA3
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	酵母细胞
5'测序引物：	根据序列设计引物
3'测序引物：	根据序列设计引物

质粒简介：
p53-AbAi is a yeast reporter vector that serves as a positive control in the Matchmaker Gold Yeast One-Hybrid Library Screening System. The vector contains a p53 binding site, located upstream of the yeast iso-1-cytochrome C minimal promoter and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A. Expression of AUR1-C, and thus AbA resistance, is induced by the binding of GAL4 activation domain-p53 fusion proteins to the p53 binding site upstream of AUR1-C. p53-AbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the nonfunctional ura3-52 locus of the yeast strain provided in the Matchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for the selection of recombinant yeast.
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