别名： XM_419615.5
原核抗性： 卡那霉素Kan
筛选标记： 遗传霉素G418
克隆菌株： 大肠杆菌DH5α
培养条件： 37℃
序列：
CDS 4018..4812
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5044..5091
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5420..6008
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct atgatagtgt ttgtcaggtt caactccagc
1381 catggcttcc cagtggagct tgggttggat gccagcatcc tccagctgaa ggaggcggtt
1441 gcgcagcgac agggagttcc agctgatcag ctgcgggtga tttttgcagg aagagagctc
1501 agcaacgacc taacactgca gaattgcgac ctggtccagc aaagcatcgt tcatattgtt
1561 cagaatctac agaagaatag tgataaggat gagacagaag acaatcatgc tggaggcatt
1621 ctaaaaacat tggaaagagt acctgaaagt ctaactcgca tagatctcag ttccagcatc
1681 cttccatcgt tgtctgcagg gttggctgtt attctggata ctaaagaacc caacatttct
1741 cctccctctg aaaaatcagg tgcagctagt tacaacagct tttatgtttt ttgcaagaat
1801 ttctgccaag ctgtgaaacc tgggaaactg agggttcgat gcaatgaatg taaacaagga
1861 acactcactt tggcaagggg cccatcttgc tgggatgatg tgctgattcc aaacagaata
1921 acaggtgttt gccagtcccc agactgcagt ggaaacgtag cggagtttta ctttaaatgt
1981 ggagcacacc caacaactga cagtgaaaca tctgtggctt tgaaccttgt tactacaaac
2041 agtcgcggta tcacatgtat aacatgcacg gatattagga gtcccgtgct tgtgttccag
2101 tgcatgcatc gccatgtcat ttgcctagat tgtttccatt tgtactgtgt gacaatgttg
2161 aatgatcgtc agttcatcta tgacccagat cttggctact ccctcccgtg tgtagctggc
2221 tgtccagact ccttgattaa agaggttcat cacttcagga ttctgggaga agaacagtac
2281 aaccgctacc agcgctatgg tgcggaggag tgcgtgctgc agatgggtgg cctgctgtgt
2341 ccaactccca gctgtggcgc aggtctgcta cctgaaccag aagtgaggaa aattgtatgt
2401 gaaccaggca atggaatagg ctgtgggttt gtgttctgcc gtgaatgtaa agaagaatac
2461 cacgaaggtg aatgcagctc tttcctcagc acacagggag ctgtggccca aaagggatat
2521 gtagttgatg aaaatgcagc catgcaagcc cggtgggaag aggcatctaa agaaacaatc
2581 aagaaaacaa ccaagccatg ccccaactgc catattccag tggagaaaaa tggtggctgc
2641 atgcacatga aatgtcctcg acctcagtgc aggtttgagt ggtgctggaa ctgtggcctg
2701 gagtggaaca gaacctgcat gggcaatcac tggtttgact gaaagcttcg aattctgcag
2761 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
2821 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
2881 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
2941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
3001 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
3061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
3121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
3181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
3241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
3301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
3361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
3421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
3481 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
3601 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
3661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
3841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
3901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
3961 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
4021 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
4081 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
4141 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
4201 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
4261 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
4321 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
4381 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
4441 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
4501 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
4561 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
4621 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
4681 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
4741 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
4801 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
4861 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
4921 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
4981 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
5041 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
5101 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
5161 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
5221 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
5281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
5581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
6121 at
