您当前的位置是:

pFastBac-GST-C质粒

  • 货号:kl-zl-1096-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1096-01 pFastBac-GST-C质粒 NA

立即咨询

+ -

pFastBac-GST-C质粒 货号:kl-zl-1096 规格:20ul

启动子: Polyhedrin
复制子: pUC ori,F1 ori
终止子: SV40 poly(A) signal
质粒分类: 昆虫细胞载体;杆状病毒组成型表达质粒
质粒大小: 5468bp
质粒标签: N-3C,N-GST
原核抗性: 氨苄青霉素Ampicillin
筛选标记: 庆大霉素Gentamicin
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 昆虫细胞
诱导方式: 组成型表达,无需诱导
5'测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3'测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
备注: 可表达GST融合蛋白
质粒简介:
The Bac-to-Bac® Baculovirus Expression System provides a rapid and efficient method to generate recombinant baculoviruses (Ciccarone et al., 1997). This method was developed by researchers at Monsanto, and is based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli (Luckow et al., 1993). The major components of the Bac-to-Bac® Baculovirus Expression System include:
• A choice of pFastBac™ donor plasmids that allow generation of an expression construct containing the gene of interest where expression of the gene of interest is controlled by a baculovirus-specific promoter.
• An E. coli host strain, DH10Bac™, that contains a baculovirus shuttle vector (bacmid) and a helper plasmid, and allows generation of a recombinant bacmid following transposition of the pFastBac™ expression construct.
质粒图谱:

货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0977-01 pMXs-miR-GFP/Puro质粒

立即咨询

+ -
kl-zl-0728-01 pCold I质粒

立即咨询

+ -
kl-zl-0747-01 pCDNA3.1-SGN质粒

立即咨询

+ -
kl-zl-0184-01 pSUMO-mut质粒

立即咨询

+ -
kl-zl-0824-01 pBV222质粒

立即咨询

+ -
kl-zl-0565-01 TAGZyme pQE-2质粒

立即咨询

+ -
KL-ZL0012-01 pLV-CiR质粒(PLCDH-Cir)

立即咨询

+ -
kl-zl-0561-01 p53-ABAI质粒

立即咨询

+ -
kl-zl-0356-01 pDD162 (Peft-3Cas9 + Empty sgRNA)质粒

立即咨询

+ -
kl-zl-0328-01 pCAS质粒(酵母细胞质粒)

立即咨询

+ -
kl-zl-0683-01 pBBR1MCS2-Tac-NgAgo质粒

立即咨询

+ -
KL-ZL-1124-01 pCold-SUMO-10×His-3C-Strep II-FLAG质粒 (pCold系列质粒

立即咨询

+ -