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pAC5.1b-EGFP质粒

货号：kl-zl-1092-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1092-01	pAC5.1b-EGFP质粒	NA		立即咨询	+ -

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pAC5.1b-EGFP质粒货号：kl-zl-1092 规格：20ul

启动子：	Ac5
复制子：	pUC ori
终止子：	SV40 poly(A) signal
质粒分类：	昆虫细胞组成性表达质粒
质粒大小：	6072bp
质粒标签：	N-EGFP，C-V5，C-6×His
原核抗性：	氨苄青霉素Ampicillin
筛选标记：	灭瘟素Blasticidin/潮霉素Hygromycin
克隆菌株：	DH5α
培养条件：	37℃，有氧，LB
表达宿主：	昆虫细胞
诱导方式：	组成性表达，无需诱导
5'测序引物：	Ac5-F：ACACAAAGCCGCTCCATCAG
3'测序引物：	BGH-R：TAGAAGGCACAGTCGAGG

质粒简介：
pAC5.1b-EGFP is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest.
• The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
• Multiple cloning site to facilitate cloning the gene of interest.
• C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
• Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
• N-terminal EGFP tag for better detection.
• Ampicillin resistance gene for selection of transformants in E. coli.
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