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pTRE2pur质粒

货号：kl-zl-0770-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0770-01	pTRE2pur质粒	NA		立即咨询	+ -

产品介绍
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pTRE2pur质粒货号：kl-zl-0770 规格：20ul

启动子：	Tet-responsive promoter
复制子：	pUC ori
终止子：	SV40 poly(A) signal，beta-globin polyA signal
质粒分类：	哺乳细胞，四环素调控系统载体
质粒大小：	5108bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	四环素诱导
5'测序引物：	Sv40-polyA-R：GAAATTTGTGATGCTATTGC
3'测序引物：	根据序列设计引物

质粒简介：
pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On® and Tet-Off® Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.
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