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pRL-CMV质粒

货号：kl-zl-1019-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1019-01	pRL-CMV质粒	NA		立即咨询	+ -

产品介绍
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pRL-CMV质粒货号：kl-zl-1019 规格：20ul

启动子：	CMV,T7
复制子：	pUC ori
终止子：	SV40 poly(A) signal
质粒分类：	哺乳细胞，信号通路报告载体
质粒大小：	4079bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	Sv40-polyA-R：GAAATTTGTGATGCTATTGC

质粒简介：
The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors： a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.
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