货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
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kl-zl-0716-01 | pTYB4质粒 | NA |
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pTYB4质粒 货号:kl-zl-0716 规格:20ul
启动子: | T7 |
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复制子: | ColE1 ori,M13 ori |
终止子: | rrnB T1,rrnB T2 terminator |
质粒分类: | 大肠杆菌载体;蛋白表达质粒 |
质粒大小: | 7474bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧,LB |
表达宿主: | 大肠杆菌 |
备注: | 表达质粒 |
pTYB4 is an E. coli expression vector (7,474 bp) used in the IMPACT™ Kit (NEB #E6901) (1). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa) (1,2). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein (3).