您当前的位置是:

pmirGLO质粒

  • 货号:kl-zl-1017-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1017-01 pmirGLO质粒 NA

立即咨询

+ -

pmirGLO质粒 货号:kl-zl-1017 规格:20ul

启动子: PGK,SV40
复制子: pUC ori,SV40 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,信号通路报告载体
质粒大小: 7350bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
3'测序引物: 根据序列设计引物
质粒简介:
The pmirGLO Dual-Luciferase miRNA Target Expression Vector(a–d) is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites 3´ of the firefly luciferase gene (luc2). These target sites can be introduced by cloning putative miRNA binding sites alone, or the 3´ untranslated region (UTR) of a gene of interest, to study the influence of these sites on transcript stability and activity. Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with firefly luciferase (luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
质粒图谱:

货号 产品名称 规格 库存 价格 数量 购买
KL-ZL-1150-01 pmKeima-Red-Mito-7质粒(荧光定位质粒)

立即咨询

+ -
KL-ZL-1130-01 pCT74质粒 (真菌表达质粒)

立即咨询

+ -
kl-zl-0622-01 pQE-4质粒

立即咨询

+ -
KL-ZL-1226-01 pEX18TC大肠自杀质粒

立即咨询

+ -
kl-zl-0840-01 pRS425质粒

立即咨询

+ -
kl-zl-0808-01 pGreenII 0800-Luc质粒

立即咨询

+ -
KL-ZL-0117-01 pET-43.1b质粒

立即咨询

+ -
KL-ZL1945-01 pENTR223-PMS1-T477N人源基因质粒

立即咨询

+ -
kl-zl-0433-01 pCXLE-hSK质粒

立即咨询

+ -
KL-Z1993-01 pDsRed2-1哺乳启动子质粒

立即咨询

+ -
kl-zl-0394-01 pEGFP-FAK质粒

立即咨询

+ -
KL-ZL-1113-01 pFN10A (ACT)质粒

立即咨询

+ -