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pShuttle-CMV质粒

  • 货号:kl-zl-0987-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0987-01 pShuttle-CMV质粒 NA

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pShuttle-CMV质粒 货号:kl-zl-0987 规格:20ul

启动子: CMV
复制子: pBR322 ori
终止子: SV40 poly(A) signal
质粒分类: 病毒系列,腺病毒载体
质粒大小: 7384bp
原核抗性: Kan
筛选标记: Neo
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
质粒简介:
he vector pShuttle-CMV contains a multiple cloning site sandwiched between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb). pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.
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