货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
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kl-zl-0275-01 | pGL4.26[luc2/minP/Hygro] 质粒 | NA |
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pGL4.26[luc2/minP/Hygro] 质粒 货号:kl-zl-0275
质粒类型: | 启动子报告载体 |
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克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5882 bp |
载体抗性: | Ampicillin (氨苄青霉素) |
筛选标记: | Hygromycin (潮霉素) |
备注: | Vector with a minimal promoter for measuring the activity of transcriptional response elements using a luciferase assay. |
The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
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