货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
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kl-zl-0640-01 | pFLAG-CMV2质粒 | NA |
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pFLAG-CMV2质粒 货号:kl-zl-0640 规格:20ul
启动子: | CMV |
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复制子: | pBR322 ori,F1 ori,SV40 ori |
终止子: | HGH poly(A)signal |
质粒分类: | 哺乳细胞载体;蛋白过表达质粒 |
质粒大小: | 4679bp |
质粒标签: | N-Flag |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧,LB |
表达宿主: | 哺乳细胞 |
5'测序引物: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | hGH-pA-R: CCAGCTTGGTTCCCAATAGA |
The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG fusion proteins in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG® -fusion constructs.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells. The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-BAP constructs.
pFLAG-CMV-2-BAP Control Plasmid is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.
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