| 货号 | 产品名称 | 规格 | 库存 | 价格 | 数量 | 购买 |
|---|---|---|---|---|---|---|
| kl-zl-0640-01 | pFLAG-CMV2质粒 | NA |
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pFLAG-CMV2质粒 货号：kl-zl-0640 规格：20ul
| 启动子： | CMV |
|---|---|
| 复制子： | pBR322 ori,F1 ori,SV40 ori |
| 终止子： | HGH poly（A）signal |
| 质粒分类： | 哺乳细胞载体;蛋白过表达质粒 |
| 质粒大小： | 4679bp |
| 质粒标签： | N-Flag |
| 原核抗性： | 氨苄青霉素Amp |
| 克隆菌株： | DH5α |
| 培养条件： | 37℃，有氧，LB |
| 表达宿主： | 哺乳细胞 |
| 5'测序引物： | CMV-F： CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物： | hGH-pA-R： CCAGCTTGGTTCCCAATAGA |
The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG fusion proteins in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG® -fusion constructs.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells. The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-BAP constructs.
pFLAG-CMV-2-BAP Control Plasmid is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.
质粒图谱：
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