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pTet-On-Advanced质粒

货号：kl-zl-0286-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0286-01	pTet-On-Advanced质粒	NA		立即咨询	+ -

产品介绍
相关产品

pTet-On-Advanced质粒货号：kl-zl-0286

质粒类型：	四环素调控系统
启动子：	CMV
克隆方法：	多克隆位点，限制性内切酶
载体大小：	7139 bp
载体抗性：	Ampicillin (氨苄青霉素)
筛选标记：	Neomycin（新霉素）

质粒简介：
pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On® System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.
质粒应用：
The pTet-On-Advanced Vector is used to develop stable Tet-On Advanced cell lines, which are hosts for a Dox-induced gene expression system. Once a vector containing a gene of interest under control of a Tet-responsive element (e.g., TRE-Tight or TRE2) is transfected into a Tet-On Advanced cell line, rtTA-Advanced binds to the TRE, and activates transcription of the gene of interest in the presence of Dox in a highly dose-dependent manner. Additional information on TRE-containing vectors and protocols describing the construction of Tet-On Advanced cell lines can be found in the Tet-On Advanced Inducible Gene Expression System User Manual (PT3898-1).
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