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pDM4大肠自杀质粒

货号：kl-zl-0692-01
培养条件：37℃，LB，有氧

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0692-01	pDM4大肠自杀质粒	NA		立即咨询	+ -

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pDM4质粒货号：kl-zl-0692 规格：5ug
一,质粒描述

复制子： R6Kγori，oriT

质粒分类：大肠系列质粒；大肠编辑质粒；大肠SacB质粒

质粒大小： 7104bp

原核抗性：氯霉素Chl（50μg/ml）

克隆菌株： S17-1γpri等大肠杆菌

培养条件： 37℃，LB，有氧

5'测序引物： Cat-R（GGTATTTATTCGGCGCAAAGTGC）

3'测序引物： Cat-F（GTGACAATCACGAAACGCGG）
二质粒简介：
pDM4质粒是一种自杀型载体，在转化的时候需要用含pri基因的菌株，复制子是oriT，质粒大小是7104bp，带有氯霉素抗性，通过抗生素抗性筛选大量复制扩增，培养条件是LB，37℃，有氧。
三,质粒序列：

promoter 3753..4198

/gene="sacR"

/note="sacB promoter"

/note="sacB promoter and control region"

CDS 4199..5620

/codon_start=1

/gene="Bacillus subtilis sacB"

/product="secreted levansucrase that renders bacterial

growth sensitive to sucrose"

/note="SacB"

/note="negative selection marker"

/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH

ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH

IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW

SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG

KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA

YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE

IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK

MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV

VKDSILEQGQLTVNK"

ORIGIN

1 gatccttttt gtccggtgtt gggttgaagg tgaagccggt cggggccgca gcgggggccg

61 gcttttcagc cttgcccccc tgcttcggcc gccgtggctc cggcgtcttg ggtgccggcg

121 cgggttccgc agccttggcc tgcggtgcgg gcacatcggc gggcttggcc ttgatgtgcc

181 gcctggcgtg cgagcggaac gtctcgtagg agaacttgac cttccccgtt tcccgcatgt

241 gctcccaaat ggtgacgagc gcatagccgg acgctaacgc cgcctcgaca tccgccctca

301 ccgccaggaa cgcaaccgca gcctcatcac gccggcgctt cttggccgcg cgggattcaa

361 cccactcggc cagctcgtcg gtgtagctct ttggcatcgt ctctcgcctg tcccctcagt

421 tcagtaattt cctgcatttg cctgtttcca gtcggtagat attccacaaa acagcaggga

481 agcagcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat

541 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt

601 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt

661 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc

721 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca

781 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag

841 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg

901 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca

961 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc

1021 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc

1081 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt

1141 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc

1201 atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc

1261 gagcgcctgg gtcacgtgcg cgtcacgaac tgcgaggcaa acaccctgcc cgctgtcatg

1321 gccgaggtga tggcgaccca gcacggcaac acccgttccg aggccgacaa gacctatcac

1381 ctgctggtta gcttccgcgc gggagagaag cccgacgcgg agacgttgcg cgcgattgag

1441 gaccgcatct gcgctgggct tggcttcgcc gagcatcagc gcgtcagtgc cgtgcatcac

1501 gacaccgaca acctgcacat ccatatcgcc atcaacaaga ttcacccgac ccgaaacacc

1561 atccatgagc cgtatcgggc ctaccgcgcc ctcgctgacc tctgcgcgac gctcgaacgg

1621 gactacgggc ttgagcgtga caatcacgaa acgcggcagc gcgtttccga gaaccgcgcg

1681 aacgacatgg agcggcacgc gggcgtggaa agcctggtcg gctggatccg gccacgatgc

1741 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc

1801 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact

1861 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca

1921 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct

1981 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg

2041 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct

2101 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt ccacatgtgg

2161 aattccacat gtggaattgt gagcggataa caatttgtgg aatcccggga gagctcaggt

2221 tacccgcatg caagatctat ctagaagggc cccactagtg acgcgtactc gagggtcgac

2281 ggtatcgata agcttgatat acactccgct agcgctgatg tccggcggtg cttttgccgt

2341 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg

2401 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca

2461 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt

2521 gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg

2581 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt

2641 gagttatcga gattttcagg agctaargaa gctaaaatgg agaaaaaaat cactggatat

2701 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt

2761 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta

2821 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat

2881 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt

2941 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa

3001 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt

3061 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat

3121 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc

3181 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg

3241 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta

3301 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc

3361 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat

3421 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg

3481 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa

3541 atgcctgagg ccagwttgct cagctctccc gtggaggtaa taattgacga tatgatcatt

3601 tattctgcct cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg

3661 ttccacaggg tagccagcag catcctgcga tgcagatcat cgaattcctg cagccaagct

3721 agacctaggc cttaagatcc tttttaaccc atcacatata cctgccgttc actattattt

3781 agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca

3841 tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct

3901 ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga

3961 ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt

4021 tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg

4081 tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa

4141 caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat

4201 gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc

4261 aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata

4321 cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga

4381 aaaatatcaa gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg

4441 cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca

4501 cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat

4561 ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg

4621 cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca

4681 agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga

4741 tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc

4801 atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga

4861 cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga

4921 caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt

4981 tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc

5041 atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga

5101 taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga

5161 ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat

5221 tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg

5281 atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc

5341 taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga

5401 tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg

5461 aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc

5521 aacgtttgcg ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga

5581 cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa gaaaatgccg

5641 atatcctatt ggcattttct tttatttctt atcaacataa aggtgaatcc catatgaact

5701 atataaaagc aggcaaatgg ctaaccgtat tcctaacctt ttggtaatga ctccaactta

5761 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt

5821 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg

5881 ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga

5941 ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct

6001 cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt

6061 ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc

6121 ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt

6181 taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata

6241 atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc

6301 gtaccgggtt gagaagcggt gtaagtgaac tgcagcaatg gcaacaacgt tgcgcaaact

6361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc

6421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga

6481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg

6541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg

6601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca

6661 agtttactca tatatacttt agattgattt atggtgcact ctcagtacaa tctgctctga

6721 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg

6781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc

6841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca

6901 tcaccgaaac gcgcgaggca gcaaggagat ggcgcccaac agtcccccgg ccacggggcc

6961 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc

7021 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc

7081 ggccacgatg cgtccggcgt agag

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