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pRS315-TEF-CYC1酿酒酵母质粒

  • 货号：KL-ZL3334-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3334-3ug pRS315-TEF-CYC1酿酒酵母质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3334 pRS315-TEF-CYC1酿酒酵母质粒

3ug

》产品描述

原核抗性： Amp 筛选标记： LEU2 克隆菌株： DH5α 培养条件： LB/37℃

》产品简介

pRS315-TEF-CYC1酿酒酵母质粒

》产品图谱及序列


序列：
promoter complement(5948..6052)
/gene="bla"
/note="AmpR promoter"
misc_feature 6089..6592
/note="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
3121 ttggagctcc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
3181 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
3241 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga
3301 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
3361 tcttgaaaat tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
3421 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
3481 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttctagaact
3541 agcgcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta
3601 tcgataccgt cgactcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
3661 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
3721 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
3781 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
3841 acgctcgaag gctttaattt gcgggcccgg tacccagctt ttgttccctt tagtgagggt
3901 taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
3961 tcacaattcc acacaacata ggagccggaa gcataaagtg taaagcctgg ggtgcctaat
4021 gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
4081 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4141 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4201 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
4261 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4321 tggcgttttt ccataggctc ggcccccctg acgagcatca caaaaatcga cgctcaagtc
4381 agaggtggcg aaacccgaca ggactataaa gataccaggc gttcccccct ggaagctccc
4441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4501 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
4561 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
4741 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
4801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
4861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
4921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactgc
5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
5461 aacgatcaag gcgagttaca tgatccccca tgttgtgaaa aaaagcggtt agctccttcg
5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6061 cccgaaaagt gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa
6121 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
6181 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
6241 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
6301 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
6361 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
6421 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
6481 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
6541 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa
6601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
6661 c

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