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pEGFP-KIF3b人源基因质粒

  • 货号：KL-ZL3370-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3370-3ug pEGFP-KIF3b人源基因质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3370 pEGFP-KIF3b人源基因质粒

3ug

》产品描述

别名： NM_004798.3 启动子： CMV 复制子： pUC 终止子： bGH 原核抗性： 卡那霉素Kan 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pEGFP-KIF3b(NM_004798.3)是一个人源基因哺乳过表达质粒。

》产品图谱及序列


序列：
CDS 4853..5647
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5879..5926
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 6255..6843
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct atgtcaaagt tgaaaagctc agagtcagtc
1381 agggtggtgg ttcgctgtcg gcccatgaat ggcaaggaaa aggctgcttc gtatgacaaa
1441 gtggtggatg tggatgttaa gctggggcag gtgtctgtga agaaccccaa agggacggcc
1501 catgaaatgc ccaagacctt cacctttgat gccgtctatg actggaatgc caagcagttt
1561 gaactgtacg atgagacgtt ccgaccactt gttgactctg tcctgcaagg tttcaatgga
1621 accatttttg cctatggaca aactgggaca ggaaaaacct acaccatgga aggaatccgt
1681 ggtgaccctg aaaaaagagg agtcattcct aactcatttg accatatctt cacccacatc
1741 tctcgatccc agaatcaaca atacctggtc agggcttctt acttagagat ctaccaggag
1801 gagatccgag atttgctctc aaaggatcag accaaaaggc ttgagctcaa agagaggcct
1861 gacacaggag tgtatgtgaa agacctgtct tcctttgtca ccaagagtgt gaaggagata
1921 gagcatgtga tgaatgtggg gaaccagaac cgttctgtcg gtgctaccaa catgaacgag
1981 cacagctcgc gttctcatgc aattttcgtt atcactattg agtgcagcga ggtgggcctc
2041 gatggtgaaa accacatccg tgtaggaaaa ttgaaccttg tagatcttgc tggcagcgaa
2101 cggcaagcca agaccggcgc acaaggggag agattaaaag aagctaccaa gatcaacctc
2161 tccctttccg ctttgggtaa tgtcatctct gctctagtgg acggcaaaag cactcacatt
2221 ccatatcggg actcaaagct taccaggctc ctccaagatt cccttggtgg caatgccaag
2281 actgtgatgg tggccaacgt ggggcctgcc tcttacaacg tagaagagac tctgaccact
2341 ctgcgatatg ccaaccgtgc caaaaacatt aagaacaaac caagggtcaa tgaggacccc
2401 aaggatgccc tccttcgaga attccaggaa gagattgctc ggctcaaggc ccagctggaa
2461 aaacggtcca ttggtaggag gaagaggcga gagaagcgga gggaaggtgg tggcagtggt
2521 gggggtgggg aagaggagga ggaggaggga gaagagggtg aggaggaagg ggatgataag
2581 gatgattact ggcgggaaca gcaagaaaaa ctggagattg agaagcgggc cattgtagag
2641 gatcacagct tggttgcaga ggagaagatg aggctgctga aggagaaaga gaaaaagatg
2701 gaggacctgc ggcgggagaa ggatgctgcc gagatgctgg gcgccaagat caaggccatg
2761 gagagtaagt tgcttgttgg aggaaaaaat atagtagatc atacgaatga acagcagaaa
2821 atcctggagc agaaacgaca ggaaattgca gagcagaaac gtcgagaaag agaaatccag
2881 caacagatgg aaagtcgaga tgaggagacc ttggaactta aagagacata cagctcattg
2941 cagcaagagg tggacatcaa gaccaaaaaa ctcaaaaagc tcttctccaa gcttcaggca
3001 gtgaaggctg agatccatga cctccaagaa gaacacatca aggagcgcca agagctagag
3061 cagactcaga atgagctcac cagggagctg aaactcaagc atcttattat agaaaacttt
3121 atccctctgg aagaaaaaag taaaattatg aatagagcct tctttgatga agaggaagat
3181 cattggaaac tacatcctat aaccagactg gagaaccagc agatgatgaa gcggccagtc
3241 tcagccgtgg gatataagag accattgagc cagcacgcaa gaatgtccat gatgattcgt
3301 ccagaggccc gatatagggc agaaaacatt gtgctgttag agctggacat gcccagccgg
3361 accaccagag actatgaggg tccagccatt gcccccaagg tccaggctgc attggatgcg
3421 gctctgcagg atgaagatga gatacaggtg gatgcatcat catttgaaag cactgcaaat
3481 aagaaatcca aggccaggcc taaaagtgga aggaagtcgg gatcctcctc ctcttcctca
3541 ggaacccctg catctcagct ttatccacag tctcgggggc tggttccaaa gtaagtcgac
3601 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
3661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
3721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
3781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
3841 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
3901 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
3961 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
4021 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
4081 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
4141 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
4201 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
4261 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
4321 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
4381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4441 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
4501 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4561 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4621 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
4681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
4741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
4801 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
4861 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
4921 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
4981 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
5041 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
5101 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
5161 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
5221 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
5281 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
5341 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
5401 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
5461 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
5521 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
5581 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
5641 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
5701 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
5761 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
5821 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
5881 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
5941 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
6001 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
6061 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
6121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
6901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat

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