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pSilencer 4.1-CMV puro-Stuffer哺乳干扰质粒

货号：KL-ZL3367-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3367-3ug	pSilencer 4.1-CMV puro-Stuffer哺乳干扰质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3367	pSilencer 4.1-CMV puro-Stuffer哺乳干扰质粒	3ug

》产品描述

原核抗性：氨苄青霉素Amp 筛选标记：嘌呤霉素Puro 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pSilencer 4.1-CMV puro-Stuffer是一个哺乳细胞干扰RNA表达质粒。

》产品图谱及序列

序列：

CDS complement(4808..5407)

/codon_start=1

/gene="pac from Streptomyces alboniger"

/product="puromycin N-acetyltransferase"

/label=PuroR

/note="confers resistance to puromycin"

/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

promoter complement(5504..5820)

/label=SV40 promoter

/note="SV40 enhancer and early promoter"

rep_origin 5518..5653

/label=SV40 ori

/note="SV40 origin of replication"

misc_feature 6235..6293

/label=CMV pA signal

misc_feature 6299..1564

/label=Stuffer

ORIGIN

1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga

61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt

121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg

181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact

241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga

301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt

361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat

421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc

481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa

541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc

601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc

661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata

721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata

781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta

841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc

901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc

961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct

1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg

1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga

1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt

1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga

1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc

1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa

1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca

1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc

1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg

1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc

1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat

1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg

1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag

1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg

1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat

1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc

1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc

2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact

2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg

2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata

2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc

2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga

2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt

2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga

2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc

2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg

2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc

2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc

2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga

2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc

2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat

2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg

2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc

3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga

3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact

3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt

3181 ggtagctctt gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag

3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg

3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa

3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata

3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg

3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata

3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg

3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct

3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt

3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc

3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga

3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt

3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc

3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa

4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca

4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca

4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct

4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc

4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa

4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt

4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc

4441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag

4501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata

4561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg

4621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat

4681 gatcctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt

4741 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg

4801 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct

4861 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg

4921 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga

4981 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc

5041 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca

5101 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt

5161 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct

5221 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga

5281 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga

5341 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact

5401 cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg

5461 gcaccggtcg tggcggccga cctgcaggca tgcaagctag ctttttgcaa aagcctaggc

5521 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct

5581 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag

5641 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca

5701 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact

5761 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc

5821 aatctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc

5881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc

5941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt

6001 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg

6061 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc

6121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt

6181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctagcggc

6241 cgcatacaaa aaaccaacac acagatccaa tgaaaataaa agatccttta ttaagcttgg

6301 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg

6361 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag

6421 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc

6481 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg

6541 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg

6601 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa

6661 cgtgaattgc tcaacagta

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