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pCDNA3.1-GNE-Myc-His人源基因质粒

  • 货号：KL-ZL3365-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3365-3ug pCDNA3.1-GNE-Myc-His人源基因质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3365 pCDNA3.1-GNE-Myc-His人源基因质粒

3ug

》产品描述

别名： NM_001128227.2 原核抗性： 氨苄青霉素Amp 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pCDNA3.1-GNE-myc-His(NM_001128227.2)是一个人源基因哺乳细胞表达质粒。

》产品图谱及序列


序列：
CDS complement(6779..7639)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=Amp
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7640..7744)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
961 accacactgg actagtggat ccgccaccat ggaaacctat ggttatctgc agagggagtc
1021 atgctttcaa ggacctcatg aactctattt taagaacctc tcaaaacgaa acaagcaaat
1081 catggagaag aatggaaata accgaaagct gcgggtttgt gttgctactt gtaaccgtgc
1141 agattattct aaacttgccc cgatcatgtt tggcattaaa accgaacctg agttctttga
1201 acttgatgtt gtggtacttg gctctcacct gatagatgac tatggaaata catatcgaat
1261 gattgaacaa gatgactttg acattaacac caggctacac acaattgtga ggggagaaga
1321 tgaggcagcc atggtggagt cagtaggcct ggccctagtg aagctgccag atgtccttaa
1381 tcgcctgaag cctgatatca tgattgttca tggagacagg tttgatgccc tggctctggc
1441 cacatctgct gccttgatga acatccgaat ccttcacatt gaaggtgggg aagtcagtgg
1501 gaccattgat gactctatca gacatgccat aacaaaactg gctcattatc atgtgtgctg
1561 cacccgcagt gcagagcagc acctgatatc catgtgtgag gaccatgatc gcatcctttt
1621 ggcaggctgc ccttcctatg acaaacttct ctcagccaag aacaaagact acatgagcat
1681 cattcgcatg tggctaggtg atgatgtaaa atctaaagat tacattgttg cactacagca
1741 ccctgtgacc actgacatta agcattccat aaaaatgttt gaattaacat tggatgcact
1801 tatctcattt aacaagcgga ccctagtcct gtttccaaat attgacgcag ggagcaaaga
1861 gatggttcga gtgatgcgga agaagggcat tgagcatcat cccaactttc gtgcagttaa
1921 acacgtccca tttgaccagt ttatacagtt ggttgcccat gctggctgta tgattgggaa
1981 cagcagctgt ggggttcgag aagttggagc ttttggaaca cctgtgatca acctgggaac
2041 acgtcagatt ggaagagaaa caggggagaa tgttcttcat gtccgggatg ctgacaccca
2101 agacaaaata ttgcaagcac tgcaccttca gtttggtaaa cagtaccctt gttcaaagat
2161 atatggggat ggaaatgctg ttccaaggat tttgaagttt ctcaaatcta tcgatcttca
2221 agagccactg caaaagaaat tctgctttcc tcctgtgaag gagaatatct ctcaagatat
2281 tgaccatatt cttgaaactc taagtgcctt ggccgttgat cttggcggga cgaacctccg
2341 agttgcaata gtcagcatga agggtgaaat agttaagaag tatactcagt tcaatcctaa
2401 aacctatgaa gagaggatta atttaatcct acagatgtgt gtggaagctg cagcagaagc
2461 tgtaaaactg aactgcagaa ttttgggagt aggcatttcc acaggtggcc gtgtaaatcc
2521 tcgggaagga attgtgctgc attcaaccaa actgatccaa gagtggaact ctgtggacct
2581 taggaccccc ctttctgaca ctttgcatct ccctgtgtgg gtagacaatg atggcaactg
2641 tgctgccctg gcggaaagga aatttggcca aggaaaggga ctggaaaact ttgttacact
2701 tatcacaggc acaggaatcg gtggtggaat tatccatcag catgaattga tccacggaag
2761 ctccttctgt gctgcagaac tgggccacct tgttgtgtct ctggatgggc ctgattgttc
2821 ctgtggaagc catgggtgca ttgaagcata cgcctctgga atggccttgc agagggaggc
2881 aaaaaagctc catgatgagg acctgctctt ggtggaaggg atgtcagtgc caaaagatga
2941 ggctgtgggt gcgctccatc tcatccaagc tgcgaaactt ggcaatgcga aggcccagag
3001 catcctaaga acagctggaa cagctttggg tcttggggtt gtgaacatcc tccataccat
3061 gaatccctcc cttgtgatcc tctccggagt cctggccagt cactatatcc acattgtcaa
3121 agacgtcatt cgccagcagg ccttgtcctc cgtgcaggac gtggatgtgg tggtttcgga
3181 tttggttgac cccgccctgc tgggtgctgc cagcatggtt ctggactaca caacacgcag
3241 gatctacaag cttgggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt
3301 cgaccatcat catcatcatc attgagttta aacggtctcc agcttaagtt taaaccgctg
3361 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
3421 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
3481 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
3541 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
3601 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
3661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
3721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
3781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
3841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
3901 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
3961 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
4021 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
4081 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
4141 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
4201 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
4261 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
4321 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
4381 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
4441 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
4501 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
4561 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
4621 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
4681 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
4741 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
4801 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
4861 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
4921 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
4981 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
5041 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
5101 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
5161 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
5221 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
5281 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
5341 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
5401 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
5461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
5521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
5581 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
5641 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
5701 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
5761 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
5821 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
7741 cgcgcacatt tccccgaaaa gtgccacctg acgtc

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