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pOJ260链霉菌质粒

货号：KL-ZL3362-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3362-3ug	pOJ260链霉菌质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3362	pOJ260链霉菌质粒	3ug

》产品描述

原核抗性：安普霉素Apr 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pOJ260是一个链霉菌基因敲除表达质粒。

》产品图谱及序列

序列：

CDS complement(169..507)

/codon_start=1

/gene="lacZ fragment"

/product="LacZ-alpha fragment of beta-galactosidase"

/label=lacZ-alpha

/translation="MITNSISRAAADPLESTCSPSLALAVVLQRRDWENPGVTQLNRLA

AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRINSPMSSTSGIG

SAADAKCR"

misc_feature 443..499

/label=MCS

promoter complement(556..586)

/label=lac promoter

/note="promoter for the E. coli lac operon"

rep_origin complement(910..1498)

/direction=LEFT

/label=ori

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS 1702..2502

/codon_start=1

/gene="aac(3)IV"

/product="aminoglycoside 3-N-acetyltransferase type IV

/label=ApmR

/note="confers resistance to apramycin (Paget and Davies,

1996)"

/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP

LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV

KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH

LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH

AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"

oriT 2947..3056

/note="incP origin of transfer"

ORIGIN

1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca

61 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt

121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt

181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga

241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct

301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa

361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg

421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgcggc

481 cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc

541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta

601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa

661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat

721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg

781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc

841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt

901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag

961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc

1021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc

1081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt

1141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt

1201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc

1261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa

1321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa

1381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg

1441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga

1501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg

1561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca

1621 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt

1681 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa

1741 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg

1801 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg

1861 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca

1921 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt

1981 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt

2041 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac

2101 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc

2161 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat

2221 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc

2281 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc

2341 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt

2401 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag

2461 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga

2521 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat

2581 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc

2641 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg

2701 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg

2761 tatcgatggt accatatatt taaagatcca ctagtatgca ggtcgacgga tcttttccgc

2821 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc

2881 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg

2941 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt

3001 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggcatg

3061 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac

3121 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg

3181 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc

3241 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg

3301 gtccgcccga gggcagagcc atgacttttt tagccgctaa acggccgggg ggtgcgcgtg

3361 attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtgaag

3421 tacatcaccg acgagcaagg caagaccgat ccccggggac ctgcatag

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