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pCU-MET3-GFP念珠菌绿色荧光质粒

货号：KL-ZL3361-3ug

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL3361-3ug	pCU-MET3-GFP念珠菌绿色荧光质粒	3ug		立即咨询	+ -

产品介绍
相关产品

》产品信息

货号	名称	规格
KL-ZL3361	pCU-MET3-GFP念珠菌绿色荧光质粒	3ug

》产品描述

原核抗性：氨苄青霉素Amp 筛选标记： URA3 克隆菌株：大肠杆菌DH5α 培养条件： 37℃

》产品简介

pCU-MET3-GFP是一个念珠菌绿色荧光表达质粒。

》产品图谱及序列

序列：

CDS complement(5797..6600)

/codon_start=1

/gene="S. cerevisiae URA3"

/product="orotidine-5'-phosphate decarboxylase, required

for uracil biosynthesis"

/label=URA3

/note="yeast auxotrophic marker, counterselectable with

5-fluoroorotic acid (5-FOA)"

/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

promoter complement(6601..6816)

/gene="S. cerevisiae URA3"

/label=URA3 promoter

ORIGIN

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

61 cttagcaggt tagactaaca tgcaaaaagt ttatattcat ttttgtagtt attcttgttt

121 tcattaggat gattacacac tgtggatgtg gattaatcga tcattgttaa tttagtgtag

181 ctactaccat ttcaaacaaa acgtaaattc ttgcagtcgt actggatctg tgaatctatt

241 agtatatatg aattaaagta gcttgtacat attattctgt tgaatcatat cgcagagcat

301 tggttgaaat ccaaaatata aaaatgtaat atcacaaaaa ataatactaa ttctaacatt

361 aatgggtcag atttttagtg aatacttaaa tttataatat cgtctattta agctagcaaa

421 tggaacaaca tttaaagtaa gaacatcata tctacatgaa aatgtatatt tcaatctgac

481 taataacgca gagcacatct ttcagtgatg tctgtcacat gatcaaaaag aattgtattt

541 aaataattca taataaaagc ttaaaaaatt acaataatga aaataaagta aataatgaca

601 tgggtaagag ttccgaaata gaatcttagt gtacaaaaca aaaattgcat cattagagat

661 ccccacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat

721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg

781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg

841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc

901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat

961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact

1021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca

1081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact

1141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg

1201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg

1261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg

1321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg

1381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag

1441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc

1501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga

1561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat

1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc

1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag

1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct

1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac

1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc

1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg

1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt

2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt

2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc

2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca

2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata

2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg

2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct

2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta

2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag

2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga

2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg

2641 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg

2701 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc

2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcatac

2821 cagttacaat tagtattaca atggtttacc aagtacaaat tatgtaatat ggttataatt

2881 ggcttatatg tatggaggta gggaactgca ggttattgtt ctgttaaact accagtaatg

2941 caagacggct aagtcatatg actgccactt tgtcaatcct gaagaaaatg acaatagaaa

3001 gtgatataat acacgtgata tgcgtaggat gggacccaaa gatagccaca tcacatgatc

3061 ttcaaaaacc ccgtcaaaaa ggaataaatc gaaggaaaaa aaatgccacg tgactttgat

3121 agtcaaaatc aggttatatt actatacgat cccccacaca taacctttac cacacaccgc

3181 acgggctgga cttatcctat tagaaagcgg tgcagccagg gccaagaaaa cgcgaacgac

3241 gcagaaaaaa cgtcaagcaa aaaaactgtg gtgttattta aagcatacaa tttcctgcct

3301 ccctttaatg ctacggggga tctagcaaat gggaaaatat cacatgactt tggctagaag

3361 gggtgggaaa acagatattt tagtcacatt gtttgatttc acgtactaca cgatacacta

3421 cactacacaa tacactgcaa tacactacac tgtatggttc cctccggccc tggatatgct

3481 agcgaaggat cccaagccca tcgaggaaat cattcaagag catctgcagg tctcaaatac

3541 actaagtcca aacacaaaca gcactgagct aatccaagac accaattgca tgccttctca

3601 attagaacag tttattacac agcttataac tgtgcatctt gccattcttt tcgagatagc

3661 catttgcatt tgattaacat gcttagctcg ttctcaagcc acagtaaaat ggattgcctt

3721 ttgagtttcc atcgttgtat aaataggcac tcattcctat agccttgctc tttggtcttg

3781 tctctgtaat ggtaatagct gtaagtcagg atataacact ccagaaaaga aacacctaac

3841 aatctagaac tagtggatcc cccgggctgc aggaattcat gtctaaaggt gaagaattat

3901 tcactggtgt tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt

3961 ctgtctccgg tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaatttattt

4021 gtactactgg taaattgccg gttccatggc caaccttagt cactactttc ggttatggtg

4081 ttcaatgttt tgctagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca

4141 tgccagaagg ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga

4201 ccagagctga agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta

4261 ttgattttaa agaagatggt aacattttag gtcacaaatt ggaatacaac tataactctc

4321 acaatgttta catcatggct gacaaacaaa agaatggtat caaagttaac ttcaaaatta

4381 gacacaacat tgaagatggt tctgttcaat tagctgacca ttatcaacaa aatactccaa

4441 ttggtgatgg tccagtcttg ttaccagaca accattactt atccactcaa tctgccttat

4501 ccaaagatcc aaacgaaaag agagaccaca tggtcttgtt agaatttgtt actgctgctg

4561 gtattaccca tggtatggat gaattgtaca aataactgca ggtcgacctc gagaacacaa

4621 cccacagcta ccaccatcaa caatatttat atatataacg tacacataga aatcacacaa

4681 acagagtatt tattcttaac tacatgaact accatcagac cgtctgggcc actatataat

4741 gtgccattca taaacgtgat cactttacgt agcaggcaac cccaggtgaa aatttttcag

4801 cgagctgcca gattgtcagg tgaaaaactg aaaaaaactt ctgggcgatg agcttgtggc

4861 gggaaattaa gtatataaag cagttagttt cttctgcttc ttgtggttct gggtattctt

4921 gtaatatttg gaagaataga aaatcaagac tactgccttt cttttcatat tatagaggaa

4981 gaaatacgca cgaacacgat atagaggtaa aggtacccaa ttcgccctat agtgagtcgt

5041 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta

5101 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg

5161 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg

5221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc

5281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg

5341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg

5401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg

5461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt

5521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt

5581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt

5641 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc

5701 ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc taatttgtga

5761 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct

5821 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc

5881 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc

5941 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt

6001 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc

6061 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt

6121 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt

6181 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc

6241 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac

6301 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga

6361 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg

6421 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt

6481 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag

6541 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat

6601 gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca

6661 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt

6721 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa

6781 tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt

6841 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac

6901 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc

6961 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga

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