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pmCherry-LC3a哺乳自噬质粒

  • 货号：KL-ZL3353-3ug
货号 产品名称 规格 库存 价格 数量 购买
KL-ZL3353-3ug pmCherry-LC3a哺乳自噬质粒 3ug

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》产品信息

货号 名称 规格
KL-ZL3353 pmCherry-LC3a哺乳自噬质粒

3ug

》产品描述

启动子： CMV 原核抗性： 卡那霉素Kan 筛选标记： 遗传霉素G418 克隆菌株： 大肠杆菌DH5α 培养条件： 37℃

》产品简介

pmCherry-LC3a是一个哺乳细胞人源基因自噬表达质粒。

》产品图谱及序列


序列：
CDS 2976..3770
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4002..4049
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4378..4966
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
1321 tccggactca gatctcgagc tatgccctca gaccggcctt tcaagcagcg gcggagcttc
1381 gccgaccgct gtaaggaggt acagcagatc cgcgaccagc accccagcaa aatcccggtg
1441 atcatcgagc gctacaaggg tgagaagcag ctgcccgtcc tggacaagac caagtttttg
1501 gtcccggacc atgtcaacat gagcgagttg gtcaagatca tccggcgccg cctgcagctg
1561 aaccccacgc aggccttctt cctgctggtg aaccagcaca gcatggtgag tgtgtccacg
1621 cccatcgcgg acatctacga gcaggagaaa gacgaggacg gcttcctcta tatggtctac
1681 gcctcccagg aaaccttcgg cttctgagaa ttctgcagtc gacggtaccg cgggcccggg
1741 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
1801 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
1861 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
1921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
1981 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
2041 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
2101 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
2161 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
2221 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
2281 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
2341 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
2401 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
2461 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
2521 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
2581 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2641 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2761 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2941 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
3001 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
3061 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
3121 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
3181 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
3241 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
3301 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
3361 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
3421 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
3481 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
3541 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
3601 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
3661 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
3721 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
3781 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
3841 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
3901 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
3961 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
4021 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
4081 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
4141 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
4201 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
4261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
4501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
4681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
4741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
4801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
4861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
4921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
4981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
5041 atcccctgat tctgtggata accgtattac cgccatgcat

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