别名： NM_004963.3；DIAR6; GC-C; GUC2C; MEC
原核抗性： Amp
筛选标记： Neo/G418
克隆菌株： DH5α
培养条件： LB/37℃
序列：
CDS complement(7678..8538)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8539..8643)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccat gaagacgttg
1021 ctgttggact tggctttgtg gtcactgctc ttccagcccg ggtggctgtc ctttagttcc
1081 caggtgagtc agaactgcca caatggcagc tatgaaatca gcgtcctgat gatgggcaac
1141 tcagcctttg cagagcccct gaaaaacttg gaagatgcgg tgaatgaggg gctggaaata
1201 gtgagaggac gtctgcaaaa tgctggccta aatgtgactg tgaacgctac tttcatgtat
1261 tcggatggtc tgattcataa ctcaggcgac tgccggagta gcacctgtga aggcctcgac
1321 ctactcagga aaatttcaaa tgcacaacgg atgggctgtg tcctcatagg gccctcatgt
1381 acatactcca ccttccagat gtaccttgac acagaattga gctaccccat gatctcagct
1441 ggaagttttg gattgtcatg tgactataaa gaaaccttaa ccaggctgat gtctccagct
1501 agaaagttga tgtacttctt ggttaacttt tggaaaacca acgatctgcc cttcaaaact
1561 tattcctgga gcacttcgta tgtttacaag aatggtacag aaactgagga ctgtttctgg
1621 taccttaatg ctctggaggc tagcgtttcc tatttctccc acgaactcgg ctttaaggtg
1681 gtgttaagac aagataagga gtttcaggat atcttaatgg accacaacag gaaaagcaat
1741 gtgattatta tgtgtggtgg tccagagttc ctctacaagc tgaagggtga ccgagcagtg
1801 gctgaagaca ttgtcattat tctagtggat cttttcaatg accagtactt tgaggacaat
1861 gtcacagccc ctgactatat gaaaaatgtc cttgttctga cgctgtctcc tgggaattcc
1921 cttctaaata gctctttctc caggaatcta tcaccaacaa aacgagactt tgctcttgcc
1981 tatttgaatg gaatcctgct ctttggacat atgctgaaga tatttcttga aaatggagaa
2041 aatattacca cccccaaatt tgctcatgct ttcaggaatc tcacttttga agggtatgac
2101 ggtccagtga ccttggatga ctggggggat gttgacagta ccatggtgct tctgtatacc
2161 tctgtggaca ccaagaaata caaggttctt ttgacctatg atacccacgt aaataagacc
2221 tatcctgtgg atatgagccc cacattcact tggaagaact ctaaacttcc taatgatatt
2281 acaggccggg gccctcagat cctgatgatt gcagtcttca ccctcactgg agctgtggtg
2341 ctgctcctgc tcgtcgctct cctgatgctc agaaaatata gaaaagatta tgaacttcgt
2401 cagaaaaaat ggtcccacat tcctcctgaa aatatctttc ctctggagac caatgagacc
2461 aatcatgtta gcctcaagat cgatgatgac aaaagacgag atacaatcca gagactacga
2521 cagtgcaaat acgacaaaaa gcgagtgatt ctcaaagatc tcaagcacaa tgatggtaat
2581 ttcactgaaa aacagaagat agaattgaac aagttgcttc agattgacta ttacaacctg
2641 accaagttct acggcacagt gaaacttgat accatgatct tcggggtgat agaatactgt
2701 gagagaggat ccctccggga agttttaaat gacacaattt cctaccctga tggcacattc
2761 atggattggg agtttaagat ctctgtcttg tatgacattg ctaagggaat gtcatatctg
2821 cactccagta agacagaagt ccatggtcgt ctgaaatcta ccaactgcgt agtggacagt
2881 agaatggtgg tgaagatcac tgattttggc tgcaattcca ttttacctcc aaaaaaggac
2941 ctgtggacag ctccagagca cctccgccaa gccaacatct ctcagaaagg agatgtgtac
3001 agctatggga tcatcgcaca ggagatcatc ctgcggaaag aaaccttcta cactttgagc
3061 tgtcgggacc ggaatgagaa gattttcaga gtggaaaatt ccaatggaat gaaacccttc
3121 cgcccagatt tattcttgga aacagcagag gaaaaagagc tagaagtgta cctacttgta
3181 aaaaactgtt gggaggaaga tccagaaaag agaccagatt tcaaaaaaat tgagactaca
3241 cttgccaaga tatttggact ttttcatgac caaaaaaatg aaagctatat ggataccttg
3301 atccgacgtc tacagctata ttctcgaaac ctggaacatc tggtagagga aaggacacag
3361 ctgtacaagg cagagaggga cagggctgac agacttaact ttatgttgct tccaaggcta
3421 gtggtaaagt ctctgaagga gaaaggcttt gtggagccgg aactatatga ggaagttaca
3481 atctacttca gtgacattgt aggtttcact actatctgca aatacagcac ccccatggaa
3541 gtggtggaca tgcttaatga catctataag agttttgacc acattgttga tcatcatgat
3601 gtctacaagg tggaaaccat cggtgatgcg tacatggtgg ctagtggttt gcctaagaga
3661 aatggcaatc ggcatgcaat agacattgcc aagatggcct tggaaatcct cagcttcatg
3721 gggacctttg agctggagca tcttcctggc ctcccaatat ggattcgcat tggagttcac
3781 tctggtccct gtgctgctgg agttgtggga atcaagatgc ctcgttattg tctatttgga
3841 gatacggtca acacagcctc taggatggaa tccactggcc tccctttgag aattcacgtg
3901 agtggctcca ccatagccat cctgaagaga actgagtgcc agttccttta tgaagtgaga
3961 ggagaaacat acttaaaggg aagaggaaat gagactacct actggctgac tgggatgaag
4021 gaccagaaat tcaacctgcc aacccctcct actgtggaga atcaacagcg tttgcaagca
4081 gaattttcag acatgattgc caactcttta cagaaaagac aggcagcagg gataagaagc
4141 caaaaaccca gacgggtagc cagctataaa aaaggcactc tggaatactt gcagctgaat
4201 accacagaca aggagagcac ctatttttaa ctcgagtcta gagggcccgt ttaaacccgc
4261 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
4321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
4381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
4441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
4501 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
4561 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
4621 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
4681 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
4741 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
4801 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
4861 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
4921 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
4981 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
5041 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
5101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
5161 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
5221 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
5281 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
5341 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
5401 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
5461 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
5521 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
5581 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
5641 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
5701 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
5761 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
5821 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
5881 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
5941 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
6001 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
6061 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
6121 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
6181 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
6241 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
6301 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
6361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6481 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
6541 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
6601 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
6661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
6721 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
6781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
6841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
6901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
6961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
7021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
7081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
7141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
7201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
7261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
7321 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
7381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
7441 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7681 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7741 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7801 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7861 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7921 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7981 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
8041 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
8101 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
8161 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
8221 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
8281 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
8341 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
8401 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
8461 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
8521 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
8581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
8641 gcgcacattt ccccgaaaag tgccacctga cgtc
