启动子： beta-Actin Promoter
复制子： pUC
原核抗性： 氨苄青霉素Amp
筛选标记： 遗传霉素G418
克隆菌株： 大肠杆菌DH5α
培养条件： 37℃
ORIGIN
1 gacggatcgg gagatcttgg ccagctgaat ggaggcccag cggcaacaca ggtcctgcct
61 ggggatcagg tctgctctgc accccacctt gctgcctgga gccgcccacc tgacaacctc
121 tcatccctgc tctgtagatc cggtcccatc cccactgccc accccacccc cccagcactc
181 cacccagttc aacgttccac gaacccccag aaccagccct catcaacagg cagcaagaag
241 ggccccccgc ccatcgcccc acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg
301 aggcagctgg caagacgcct gcagctgaaa gatacaaggc cagggacagg acagtcccat
361 ccccaggagg cagggagtat acaggctggg gaagtttgcc cttgcgtggg gtggtgatgg
421 aggaggctca gcaagtcttc tggactgtga acctgtgtct gccactgtgt gctgggtggt
481 ggtcatcttt cccaccaggc tgtggcctct gcaaccttca agggaggagc aggtcccatt
541 ggctgagcac agccttgtac cgtgaactgg aacaagcagc ctccttcctg gccacaggtt
601 ccatgtcctt atatggactc atctttgcct attgcgacac acactcaatg aacacctact
661 acgcgctgca aagagccccg caggcctgag gtgcccccac ctcaccactc ttcctatttt
721 tgtgtaaaaa tccagcttct tgtcaccacc tccaaggagg gggaggagga ggaaggcagg
781 ttcctctagg ctgagccgaa tgcccctctg tggtcccacg ccactgatcg ctgcatgccc
841 accacctggg tacacacagt ctgtgattcc cggagcagaa cggaccctgc ccacccggtc
901 ttgtgtgcta ctcagtggac agacccaagg caagaaaggg tgacaaggac agggtcttcc
961 caggctggct ttgagttcct agcaccgccc cgcccccaat cctctgtggc acatggagtc
1021 ttggtcccca gagtccccca gcggcctcca gatggtctgg gagggcagtt cagctgtggc
1081 tgcgcatagc agacatacaa cggacggtgg gcccagaccc aggctgtgta gacccagccc
1141 ccccgccccg cagtgcctag gtcacccact aacgccccag gcctggtctt ggctgggcgt
1201 gactgttacc ctcaaaagca ggcagctcca gggtaaaagg tgccctgccc tgtagagccc
1261 accttccttc ccagggctgc ggctgggtag gtttgtagcc ttcatcacgg gccacctcca
1321 gccactggac cgctggcccc tgccctgtcc tggggagtgt ggtcctgcga cttctaagtg
1381 gccgcaagcc acctgactcc cccaacacca cactctacct ctcaagccca ggtctctccc
1441 tagtgaccca cccagcacat ttagctagct gagccccaca gccagaggtc ctcaggccct
1501 gctttcaggg cagttgctct gaagtcggca agggggagtg actgcctggc cactccatgc
1561 cctccaagag ctccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac
1621 cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg
1681 cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg
1741 aaccgggcac tgcccagcgt ggggcgcggg ggccacggcg cgcgccccca gcccccgggc
1801 ccagcacccc aaggcggcca acgccaaaac tctccctcct cctcttcctc aatctcgctc
1861 tcgctctttt tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc
1921 ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc
1981 ttttatggct cgagcggccg cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc
2041 accgccgaga ccgcgtccgc cccgcgagca cagagcctcg cctttgccga tccgccgccc
2101 gtccacaccc gccgccaggt aagcccggcc agccgaccgg ggcatgcggc cgcggcccct
2161 tcgcccgtgc agagccgccg tctgggccgc agcggggggc gcatgggggg ggaaccggac
2221 cgccgtgggg ggcgcgggag aagcccctgg gcctccggag atgggggaca ccccacgcca
2281 gttcggaggc gcgaggccgc gctcgggagg cgcgctccgg gggtgccgct ctcggggcgg
2341 gggcaaccgg cggggtcttt gtctgagccg ggctcttgcc aatggggatc gcagggtggg
2401 cgcggcgtag cccccgccag gcccggtggg ggctggggcg ccattgcgcg tgcgcgctgg
2461 tcctttgggc gctaactgcg tgcgcgctgg gaattggcgc taattgcgcg tgcgcgctgg
2521 gactcaaggc gctaattgcg cgtgcgttct ggggcccggg gtgccgcggc ctgggctggg
2581 gcgaaggcgg gctcggccgg aaggggtggg gtcgccgcgg ctcccgggcg cttgcgcgca
2641 cttcctgccc gagccgctgg ccgcccgagg gtgtggccgc tgcgtgcgcg cgcgccgacc
2701 cggcgctgtt tgaaccgggc ggaggcgggg ctggcgcccg gttgggaggg ggttggggcc
2761 tggcttcctg ccgcgcgccg cggggacgcc tccgaccagt gtttgccttt tatggtaata
2821 acgcggccgg cccggcttcc tttgtcccca atctgggcgc gcgccggcgc cccctggcgg
2881 cctaaggact cggcgcgccg gaagtggcca gggcgggggc gacctcggct cacagcgcgc
2941 ccggctattc tcgcagctca agcttccacc atggtgagca agggcgagga gctgttcacc
3001 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
3061 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
3121 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
3181 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
3241 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
3301 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
3361 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
3421 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
3481 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
3541 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
3601 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
3661 actctcggca tggacgagct gtacaaggga tccactagtg gcatggatga tgatatcgcc
3721 gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc
3781 ccccgggccg tcttcccctc catcgtgggg cgccccaggc accagggcgt gatggtgggc
3841 atgggtcaga aggattccta tgtgggcgac gaggcccaga gcaagagagg catcctcacc
3901 ctgaagtacc ccatcgagca cggcatcgtc accaactggg acgacatgga gaaaatctgg
3961 caccacacct tctacaatga gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc
4021 gaggcccccc tgaaccccaa ggccaaccgc gagaagatga cccagatcat gtttgagacc
4081 ttcaacaccc cagccatgta cgttgctatc caggctgtgc tatccctgta cgcctctggc
4141 cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt gcccatctac
4201 gaggggtatg ccctccccca tgccatcctg cgtctggacc tggctggccg ggacctgact
4261 gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac ggccgagcgg
4321 gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg ccctggactt cgagcaagag
4381 atggccacgg ctgcttccag ctcctccctg gagaagagct acgagctgcc tgacggccag
4441 gtcatcacca ttggcaatga gcggttccgc tgccctgagg cactcttcca gccttccttc
4501 ctgggcatgg agtcctgtgg catccacgaa actaccttca actccatcat gaagtgtgac
4561 gtggacatcc gcaaagacct gtacgccaac acagtgctgt ctggcggcac caccatgtac
4621 cctggcattg ccgacaggat gcagaaggag atcactgccc tggcacccag cacaatgaag
4681 atcaagatca ttgctcctcc tgagcgcaag tactccgtgt ggatcggcgg ctccatcctg
4741 gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc aggagtatga cgagtccggc
4801 ccctccattg tccaccgcaa atgcttctag gaattctgca gatatccatc acactggcgg
4861 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
4921 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
4981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
5041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
5101 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
5161 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
5221 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
5281 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
5341 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct
5401 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
5461 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
5521 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat
5581 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
5641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat
5701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
5761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
5821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
5881 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
5941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
6001 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
6061 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
6121 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
6181 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
6241 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
6301 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
6361 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
6421 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
6481 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
6541 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
6601 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
6661 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
6721 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
6781 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
6841 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
6901 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
6961 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
7021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
7081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
7141 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
7201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
7261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
7321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
7381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
7441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
7501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
7561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
7621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
7681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
7741 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
7801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
7861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
7921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
7981 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
8041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
8101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
8161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
8221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
8281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
8341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
8401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
8461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
8521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
8581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
8641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
8701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
8761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
8821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
8881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
8941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
9001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
9061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
9121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
9181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
9241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
9301 gttccgcgca catttccccg aaaagtgcca cctgacgtc
