终止子： NOS terminator
质粒大小： 11735bp
质粒标签： GUS
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCXGUS-P质粒是一个植物启动子活性检测载体，通过GUS的染色强度来检测启动子活性高低。使用XcmI酶切空载体得到T载体，可连接带A的PCR产物，并在ccdb致死基因的帮助下，基因克隆的阳性率得以大大提高。
序列：
ORIGIN
1 gaattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
61 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
121 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
181 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
241 ttgccaaatg tttgaacgat cggggaaatt cgagctctca ttgtttgcct ccctgctgcg
301 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
361 gtttgcggtc gcgagtgaag atccctttct tgttaccgcc aacgcgcaat atgccttgcg
421 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
481 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
541 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
601 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
661 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
721 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
781 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
841 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
901 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
961 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
1021 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
1081 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
1141 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
1201 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
1261 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
1321 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
1381 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
1441 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
1501 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
1561 agagcattac gctgcgatgg attccggcat agttaaagaa atcatggaag taagactgct
1621 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
1681 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
1741 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
1801 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
1861 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
1921 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
1981 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
2041 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacatg gatccccaat
2101 acttgtatgg ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
2161 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
2221 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
2281 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
2341 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
2401 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
2461 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
2521 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
2581 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
2641 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
2701 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
2761 atacacagcc agtctgcagg tcgacccata caagtattgg ggatcctcta gagtcgacct
2821 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
2881 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
2941 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
3001 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
3061 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
3121 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
3181 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
3241 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
3301 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
3361 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
3421 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
3481 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
3541 ccgcccggag ctggccagga tgcttgacca cctagccctg gcgacgttgt gacagtgacc
3601 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
3661 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
3721 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
3781 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
3841 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
3901 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
3961 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
4021 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
4081 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
4141 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
4201 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
4261 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
4321 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
4381 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
4441 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
4501 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
4561 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
4621 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
4681 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gcaaccgccg acctggtgga
4741 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
4801 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
4861 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
4921 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
4981 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
5041 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
5101 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
5161 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
5221 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
5281 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
5341 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
5401 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
5461 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
5521 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
5581 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
5641 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
5701 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
5761 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
5821 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
5881 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
5941 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
6001 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
6061 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
6121 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
6181 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
6241 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
6301 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
6361 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
6421 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
6481 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
6541 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
6601 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
6661 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
6721 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
6781 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
6841 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
6901 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
6961 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
7021 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
7081 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
7141 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
7201 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
7261 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
7321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
7381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
7441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
7501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
7561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
7621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
7681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
7741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
7801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
7861 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
7921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
7981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
8041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
8101 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
8161 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
8221 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
8281 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
8341 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
8401 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
8461 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
8521 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
8581 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
8641 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
8701 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gttgtccctt
8761 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
8821 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
8881 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
8941 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
9001 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
9061 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
9121 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
9181 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
9241 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
9301 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
9361 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
9421 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
9481 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
9541 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
9601 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
9661 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
9721 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
9781 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
9841 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
9901 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
9961 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
10021 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
10081 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
10141 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
10201 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
10261 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
10321 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
10381 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
10441 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
10501 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
10561 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
10621 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
10681 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
10741 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa
10801 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
10861 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
10921 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
10981 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
11041 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
11101 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
11161 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
11221 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
11281 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
11341 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
11401 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
11461 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
11521 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
11581 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
11641 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
11701 aatttcacac aggaaacagc tatgaccatg attac
