启动子： CaMV35S promoter
终止子： NOS terminator
质粒大小： 11229bp
质粒标签： mCherry，ccdb
原核抗性： 卡那霉素Kan
筛选标记： 潮霉素Hyg
克隆菌株： 大肠杆菌DB3.1
培养条件： 37℃，有氧，LB
pCX-DR质粒是一个植物过表达质粒，35S启动子驱动DsRed2红色荧光蛋白和目的基因融合表达。使用XcmI酶切空载体得到T载体，可连入带A的PCR产物，并ccdb致死基因的作用下，克隆基因变得更加容易，阳性率高。
序列：
ORIGIN
1 gaattcgagc tcggtaccca ctggattttg gttttaggaa ttagaaattt tattgataga
61 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
121 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
181 tcgaaattcg agctccaccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc
241 caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt
301 ctccgctcat gatccgggga attaattccg atcctatctg tcacttcatc aaaaggacag
361 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcattc
421 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
481 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
541 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
601 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca agatcctcta
661 gcgctaccgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc
721 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
781 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
901 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
961 cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc ctccctgcag
1021 gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc
1081 gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
1141 ggcgtgctga agggcgagac ccacaaggcc ctgaagctga aggacggcgg ccactacctg
1201 gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac
1261 gtggacgcca agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac
1321 gagcgcaccg agggccgcca ccacctgttc ctgagatctc gagctcaagc ttcgaattct
1381 gcagtcgacg gtaccgcggg cccgggatcc ccaatacttg tatgggtcga cctgcagact
1441 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
1501 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
1561 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
1621 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
1681 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
1741 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
1801 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
1861 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
1921 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
1981 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
2041 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
2101 ccatacaagt attggggatc caccggatct agataactga tcataatcag ccataccaca
2161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
2221 aaaatgaatg caattgttgt tgttgtgtga attacaggtg accagctcga atttccccga
2281 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
2341 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
2401 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
2461 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
2521 gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta
2581 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
2641 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
2701 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
2761 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
2821 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
2881 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
2941 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
3001 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
3061 accacctagc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
3121 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
3181 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
3241 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
3301 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
3361 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
3421 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
3481 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
3541 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
3601 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
3661 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
3721 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
3781 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
3841 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
3901 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
3961 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
4021 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
4081 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
4141 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
4201 cttacgacat atgggcaacc gccgacctgg tggagctggt taagcagcgc attgaggtca
4261 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
4321 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
4381 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
4441 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
4501 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
4561 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
4621 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
4681 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
4741 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
4801 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
4861 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
4921 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
4981 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
5041 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
5101 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
5161 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
5221 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
5281 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
5341 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
5401 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
5461 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
5521 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
5581 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
5641 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
5701 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
5761 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
5821 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
5881 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
5941 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
6001 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
6061 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
6121 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
6181 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
6241 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
6301 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
6361 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
6421 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
6481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
6541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
6601 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
6661 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
6721 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
6781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
6841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
6901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
6961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
7381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
7501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
7561 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
7621 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
7681 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
7741 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
7801 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
7861 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
7921 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
7981 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
8041 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
8101 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
8161 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
8221 tccttttccc gttccacatc ataggttgtc cctttatacc ggctgtccgt catttttaaa
8281 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
8341 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
8401 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
8461 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
8521 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
8581 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
8641 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
8701 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
8761 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
8821 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
8881 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
8941 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
9001 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
9061 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
9121 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
9181 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
9241 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
9301 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
9361 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
9421 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
9481 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
9541 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
9601 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
9661 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
9721 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
9781 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
9841 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
9901 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
9961 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
10021 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
10081 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
10141 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat ctgcgaaagc
10201 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
10261 atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct
10321 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
10381 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
10441 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
10501 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
10561 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
10621 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
10681 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
10741 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
10801 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
10861 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
10921 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
10981 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
11041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
11101 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
11161 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
11221 catgattac
