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R777-E011 Hs.ARHGAP35(NM_004491.4)人源基因质粒

货号：KL-ZL-1199-01
培养条件：37℃

货号	产品名称	规格	库存	价格	数量	购买
KL-ZL-1199-01	R777-E011 Hs.ARHGAP35(NM_004491.4)人源基因质粒	NA		立即咨询	+ -

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R777-E011 Hs.ARHGAP35(NM_004491.4)人源基因质粒货号：KL-ZL-1199 规格：5ug
一,质粒描述：

别名： NM_004491.4，GRF-1; GRLF1; P190-A; P

原核抗性：壮观霉素Spe

克隆菌株：大肠杆菌DH5α

培养条件： 37℃

5'测序引物： M13F

3'测序引物： M13R
二,质粒简介：
Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)
三,质粒序列及图谱：

序列：

terminator 7081..7167

/gene="Escherichia coli rrnB"

/label=rrnB T1 terminator

/note="transcription terminator T1 from the E. coli rrnB

gene"

ORIGIN

1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa

61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt

121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg

181 gcaccatgat gatggcaaga aagcaagatg tccgaattcc cacctacaac atcagtgtgg

241 tgggattatc tgggaccgag aaggaaaagg gccagtgtgg gattggaaag tcttgtttgt

301 gcaaccgctt cgtgcgcccg agtgctgacg agtttcactt ggaccatacc tccgtcctca

361 gcaccagtga ctttggaggg cgagtggtca ataatgacca ctttctctac tggggagaag

421 ttagccgctc cctggaggat tgtgtggaat gtaagatgca cattgtggag cagactgaat

481 ttattgatga tcagactttt caacctcatc gaagcacggc cctgcagccc tatatcaaga

541 gagctgctgc gaccaagctt gcatcagctg aaaaactcat gtacttttgc actgaccagc

601 tggggctgga gcaggacttt gagcagaaac aaatgccaga cggaaagctg ctggttgatg

661 gttttcttct tggtattgat gttagcaggg gcatgaatag gaactttgat gaccagctca

721 agtttgtctc caatctctac aatcagcttg caaaaacaaa aaagcccata gtggtggtcc

781 tgactaagtg tgacgaaggt gttgagcggt acattagaga tgcacatact tttgccttaa

841 gcaaaaagaa cctccaggtt gtggagacct cagcgagatc caatgtaaac gtggacttgg

901 ctttcagcac cttagtgcaa ctcattgata aaagtcgggg aaagacaaaa atcattcctt

961 attttgaagc tctcaagcag cagagtcagc agatagctac agcaaaagac aagtatgagt

1021 ggctggtgag tcgcattgtg aaaaaccaca atgagaactg gctgagtgtc agccgaaaga

1081 tgcaggcctc tccagaatac caggactatg tctacctgga agggactcag aaagccaaga

1141 agctgtttct acagcacatc caccgcctca agcatgagca tatcgagcgt aggagaaagc

1201 tgtacctggc agccctgcca ttagcttttg aagctcttat acctaatcta gatgaaatag

1261 accacctaag ctgcataaaa gccaaaaagc tcttagaaac caagccagaa ttcttgaagt

1321 ggtttgttgt gcttgaagag accccatggg atgccaccag tcacattgac aacatggaaa

1381 acgaacggat tccctttgat ttaatggata ccgtccctgc agagcagcta tacgaggccc

1441 acttagagaa gctgaggaac gaaaggaaaa gagttgagat gcgaagggcg tttaaagaaa

1501 acctggagac ttctcctttc ataactcccg gaaagccttg ggaagaggcc cgtagtttta

1561 ttatgaatga ggatttctac cagtggctgg aggaatctgt atacatggat atttatggca

1621 aacaccaaaa gcaaattata gataaagcaa aggaagaatt tcaggagttg cttttggaat

1681 attcagaatt gttttatgaa ctggagctgg atgctaagcc cagcaaggag aagatgggtg

1741 ttattcagga tgttctggga gaggaacagc gatttaaagc attacaaaag ctccaagcag

1801 agcgtgatgc ccttattctg aaacacattc attttgtgta ccacccaaca aaggagacat

1861 gccccagctg cccagcttgt gtggacgcta agattgagca cttgattagt tctcggttta

1921 tccggccgtc tgaccggaat cagaaaaatt cactctctga ccctaacatt gatagaatca

1981 acttggttat attgggcaaa gacggccttg cccgagagtt ggccaatgag attcgagctc

2041 tttgtacaaa tgatgacaag tatgtgatag atggtaaaat gtatgagctt tccctgaggc

2101 caatagaggg gaatgtcagg cttcctgtga actctttcca gacgccaaca tttcagcccc

2161 acggctgtct ctgcctttac aattcaaagg aatcgctatc ctatgtagtg gaaagtatag

2221 agaagagtag agagtccacg ctgggccggc gggataatca tttagtccat ctccccctta

2281 cattaatttt ggttaacaag agaggagaca ccagtggaga gactctgcat agcttaatac

2341 agcaaggtca acaaattgct agcaaacttc agtgtgtctt tctcgaccct gcttctgctg

2401 gcattggtta cggacgcaac attaatgaaa agcaaatcag tcaagttttg aagggactcc

2461 tggactctaa gcgtaactta aacctggtca gttctactgc tagcatcaaa gatttggctg

2521 atgttgatct gcgaattgtt atgtgtctga tgtgtggaga tccttttagt gcagatgaca

2581 tactttttcc tgtccttcag tcccaaacct gtaaatcttc ccattgtgga agcaacaact

2641 ctgttttact tgaactacca atcggactgc acaagaagcg gattgaactg tctgttcttt

2701 cataccattc ctcctttagc atcagaaaga gccggttggt tcatgggtac attgtttttt

2761 attcagccaa acgtaaggcc tctttggcta tgttacgtgc ctttctttgt gaagtgcagg

2821 atattatccc tattcagctt gtagcactca ctgatggcgc tgtagatgtc ctggacaatg

2881 acttaagtag ggaacagcta actgaggggg aggagattgc tcaagaaatt gacggaaggt

2941 tcacaagcat cccctgtagc caaccccagc ataaacttga gatctttcac ccatttttta

3001 aagatgtggt ggaaaaaaag aacataatcg aggctactca tatgtacgat aatgctgccg

3061 aggcctgtag caccaccgaa gaggtgttta actccccccg ggcaggatca ccgctctgca

3121 actcaaacct gcaggattca gaagaagata tcgagccatc ttacagcctg tttcgagaag

3181 acacatcact gccttctctg tccaaagacc attctaagct ctctatggaa ctggagggaa

3241 atgatgggct gtctttcatt atgagcaatt ttgagagtaa actgaacaac aaagtacctc

3301 cgccagtcaa accaaagcct cctgtccatt ttgaaattac aaagggggat ctatcttatt

3361 tagaccaagg ccatagggat ggacagagga agtctgtgtc ttctagcccc tggctgcctc

3421 aggatgggtt tgatccttct gactatgctg aacccatgga tgctgtggtg aagccaagga

3481 atgaagaaga aaacatatac tccgtgcccc atgacagcac ccaaggcaaa atcatcacca

3541 ttcggaatat caacaaagcc cagtccaacg gcagcgggaa tggttctgac agtgaaatgg

3601 acaccagctc tctagagcga gggcgcaagg tttccatcgt gagcaagcca gtgctgtaca

3661 ggacgagatg cacccggctg gggcggtttg ctagttaccg gaccagcttc agcgtgggga

3721 gtgatgatga gctggggccc atccggaaga aagaggagga tcaggcatcc cagggttata

3781 aaggggacaa tgctgtcatt ccatacgaaa cagacgaaga cccgcggagg aggaatattc

3841 ttcgcagcct aaggaggaac actaagaaac caaagcccaa accccggcca tccatcacaa

3901 aggcaacctg ggagagtaac tattttgggg tgcccttaac aactgtcgtg actccagaga

3961 agccgatccc catttttatt gaaagatgta ttgagtacat tgaagccaca ggactgagca

4021 cggaaggcat ctaccgggtc agcgggaaca agtctgagat ggagagtctg cagagacagt

4081 ttgatcaaga ccacaacctg gacctggcag agaaagactt tacggtgaat accgtggctg

4141 gtgccatgaa gagctttttc tcagaactgc ctgaccccct ggtcccgtat aacatgcaga

4201 tcgacttggt ggaagcacac aaaatcaacg accgggagca gaagttgcat gcccttaagg

4261 aggtattaaa gaaatttcca aaggaaaacc acgaagtctt caagtatgtc atctctcacc

4321 taaacaaggt cagccacaac aacaaggtga atctcatgac cagcgagaac ctctccatct

4381 gcttctggcc caccttgatg agacctgatt tcagcactat ggacgccctc acagccacgc

4441 gcacctacca gacaatcatt gaactcttta tccagcagtg ccccttcttc ttctacaatc

4501 ggcccatcac cgagcccccc ggcgccaggc ccagctcccc ctctgccgtg gcttccaccg

4561 tccccttcct cacttccacg cctgtcacaa gtcagccgtc gcccccacag tcgcctccac

4621 ccacccccca gtccccaatg cagccactgc ttccctccca gcttcaagcc gaacacacgc

4681 tgtaatcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt

4741 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc

4801 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac

4861 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta

4921 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca

4981 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac

5041 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt

5101 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta

5161 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg

5221 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg

5281 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa

5341 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg

5401 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt

5461 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta

5521 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac

5581 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg

5641 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg

5701 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca

5761 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc

5821 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct

5881 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag

5941 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt

6001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc

6061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc

6121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac

6181 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac

6241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt

6301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct

6361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat

6421 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt

6481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg

6541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt

6601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt

6661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg

6721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg

6781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc

6841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg

6901 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc

6961 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg

7021 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg

7081 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc

7141 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg

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