备注：
BT007332.1
二,质粒简介：
pENTR223-NBR2是一个人源基因克隆质粒。
三,质粒序列及图谱：
序列：
rep_origin 2381..2969
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agatgtggaa aggaggtaga agtcatcctt tcctccccca tagcagcagg tgtgcaggct
721 ctggtggtca gctggactcc atactccccc accagtcacc agcctgggga ccgtggggct
781 gcaaggacct cagcagcggt ttcccaagtt tcctgacttc ttccatcctc tggaaatcag
841 ctgtggttcg tgagccacac atctccccac accaagctcc tccatacaag acctcggact
901 gcatcacgta cacaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
961 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
1021 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
1081 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
1141 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
1201 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
1261 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
1321 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
1381 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
1441 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
1501 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
1561 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
1621 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
1681 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
1741 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
1801 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
1861 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
1921 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
1981 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
2041 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
2101 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
2161 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
2221 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
2281 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
2761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
3001 tggccttttg ctggcctttt gctcacatgt t
