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pVL1392质粒

货号：kl-zl-1093-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1093-01	pVL1392质粒	NA		立即咨询	+ -

产品介绍
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pVL1392质粒货号：kl-zl-1093 规格：20ul

启动子：	Polyhedrin
复制子：	ColE1 ori
终止子：	polyhedrin 3
质粒分类：	昆虫细胞杆状病毒组成型表达质粒
质粒大小：	9032bp
原核抗性：	氨苄青霉素Ampicillin
克隆菌株：	DH5α
培养条件：	37℃，有氧，LB
表达宿主：	昆虫细胞
诱导方式：	组成性表达，无需诱导
5'测序引物：	Polyhedrin-F：AAATGATAACCATCTCGC
3'测序引物：	Polyhedrin-R：GTCCAAGTTTCCCTG

质粒简介：
pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.
• Multiple cloning site polylinker, positioned in opposite orientations
• Recombination sequences for insertion into the baculovirus genome
• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein Features for growth and maintenance in E. coli：
• Ampicillin gene for selection
• pMB1 origin of replication from pUC19 for high copy number growth
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