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pAN8-1质粒

  • 货号:kl-zl-1077-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1077-01 pAN8-1质粒 NA

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pAN8-1质粒 货号:kl-zl-1077 规格:20ul

启动子: gpdA
复制子: pUC ori
终止子: TRPC
质粒分类: 其他宿主载体;丝状真菌表达质粒
质粒大小: 6089bp
原核抗性: 氨苄青霉素Ampicillin
筛选标记: 博来霉素Bleocin(Zeocin)
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 丝状真菌
诱导方式: 无需诱导
5'测序引物: 根据全序列设计
3'测序引物: 根据全序列设计
备注: NCBI号:Z32751.1
质粒简介:
pAN8-1, encoding phleomycin resistance. Protoplasts of a vigorous Phleor transformant were then retransformed using the partially homologous vector, pAN7-1 which encodes hygromycin B resistance. Retransformation of this strain to hygromycin resistance occurred at frequencies that were consistently twofold higher than with the original recipient strain. Linearised pAN7-1 DNA transformed phleomycin-resistant protoplasts at higher frequencies still.
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