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pEYFP-Golgi质粒

  • 货号:kl-zl-1055-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1055-01 pEYFP-Golgi质粒 NA

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pEYFP-Golgi质粒 货号:kl-zl-1055 规格:20ul

启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,荧光蛋白报告载体
质粒大小: 4918bp
原核抗性: Kan
筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物
质粒简介:
pEYFP-Golgi encodes a fusion protein consisting of enhanced yellow fluorescent protein (EYFP) and a sequence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to the trans-medial region of the Golgi apparatus. The EYFP gene contains the four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 514 nm, the Em of EYFP is 84,500 cm-1M-1 and the fluorescence quantum yield is 0.61, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that of EGFP.
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