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p3xFLAG-CMV-13质粒

  • 货号:kl-zl-1038-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-1038-01 p3xFLAG-CMV-13质粒 NA

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p3xFLAG-CMV-13质粒 货号:kl-zl-1038 规格:20ul

启动子: CMV,SV40,T7
复制子: F1 ori , pUC ori
终止子: SV40 poly(A) signal,hGH poly(A) signal
质粒分类: 哺乳细胞,蛋白过表达载体
质粒大小: 6362bp
原核抗性: Amp
筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: hGH-PA-R:CCAGCTTGGTTCCCAATAGA
质粒简介:
The p3XFLAG-CMV-13 Expression Vector is a 6.3kb derivative of pCMV5 used to establish expression of transient or unstable C-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG sequence. The aminoglycoside phosphotransferase II gene (Neo) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants. p3XFLAG-CMV-13 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host, such as COS cells.
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