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Laconic/pcDNA3.1(-)质粒

货号：kl-zl-1004-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-1004-01	Laconic/pcDNA3.1(-)质粒	NA		立即咨询	+ -

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Laconic/pcDNA3.1(-)质粒货号：kl-zl-1004 规格：20ul

启动子：	CMV,T7，SV40
复制子：	pUC ori,f1 ori,SV40 ori
终止子：	SV40 poly(A) signal,bGH poly(A) signal
质粒分类：	哺乳细胞，乳酸生物传感器
质粒大小：	7648bp
原核抗性：	Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	BGH：TAGAAGGCACAGTCGAGG

质粒简介：
Lactate is shuttled between and inside cells, playing metabolic and signaling roles in healthy tissues. Lactate is also a harbinger of altered metabolism and participates in the pathogenesis of inflammation, hypoxia/ischemia, neurodegeneration and cancer. Many tumor cells show high rates of lactate production in the presence of oxygen, a phenomenon known as the Warburg effect, which has diagnostic and possibly therapeutic implications. In this article we introduce Laconic, a genetically-encoded Forster Resonance Energy Transfer (FRET)-based lactate sensor designed on the bacterial transcription factor LldR. Laconic quantified lactate from 1 µM to 10 mM and was not affected by glucose, pyruvate, acetate,
质粒图谱：

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