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pAdEasy-1质粒

货号：kl-zl-0988-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0988-01	pAdEasy-1质粒	NA		立即咨询	+ -

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pAdEasy-1质粒货号：kl-zl-0988 规格：20ul

复制子：	pUC ori
质粒分类：	病毒系列，腺病毒载体
质粒大小：	33477bp
原核抗性：	Amp
克隆菌株：	Stbl3
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	M13F：TGTAAAACGACGGCCAGT
3'测序引物：	根据序列设计引物

质粒简介：
The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.
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