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pYES2/CT质粒

  • 货号:kl-zl-0863-01
货号 产品名称 规格 库存 价格 数量 购买
kl-zl-0863-01 pYES2/CT质粒 NA

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pYES2/CT质粒 货号:kl-zl-0863 规格:20ul

启动子: URA3 ,T7,GAL1
复制子: 2μ ori,ori,FI ori
终止子: CYC1
质粒分类: 酵母系列,酿酒酵母表达载体
质粒大小: 5963bp
原核抗性: Amp
筛选标记: URA3
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 酵母细胞
诱导方式: 半乳糖诱导
5'测序引物: GAL1-F: ATTTTCGGTTTGTATTACTTC
3'测序引物: GALI-R: GTTCTTAATACTAACATAACT
质粒简介
The pYC2/CT vector contains the CEN6/ARSH4 sequence (Sikorski and Hieter, 1989) for maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski and Hieter, 1989). The CEN6 sequence is derived from the CEN6 locus of yeast chromosome 6 (Panzeri and Philippsen, 1982) while the ARSH4 sequence is derived from the yeast histone H4-associated ARS (Bouton and Smith, 1986). When placed in a heterologous expression plasmid (i.e. pYC2/CT), the presence of the CEN6/ARSH4 sequence allows non-integrative centromeric maintenance and low copy number replication of the plasmid (generally 1–2 copies per cell).
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