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pBAD18质粒

货号：kl-zl-0725-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0725-01	pBAD18质粒	NA		立即咨询	+ -

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pBAD18质粒货号：kl-zl-0725 规格：20ul

动子：	araBad
复制子：	PBR322 ori
质粒分类：	大肠杆菌载体；pBAD系列表达质粒
质粒大小：	4613bp
原核抗性：	氨苄青霉素Amp
克隆菌株：	Top10
培养条件：	37℃，有氧，LB
表达宿主：	推荐LMG194
诱导方式：	阿拉伯糖
5'测序引物：	pBAD-F： ATGCCATAGCATTTTTATCC
3'测序引物：	pBAD-R： GATTTAATCTGTATCAGG

质粒简介：
This vector is one of several tightly controlled expression vectors regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning site and need for translation initiation sequences. Cultures should be grown inminimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining media by the addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two recommended hosts differ in terms of induction characteristics, especially when using low concentrations of inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of cloned inserts： 5 primer (278bp upstream of the NheI site) ：5CTGTTTCTCCATACCCGTT 3and one of two 3primers：3 primer 1 (219bp downstream of the HindIII site)：5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of the HindIII site)：5 GGCTGAAAATCTTCTCT3
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