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pCDNA3.1(+)/Myc-His B质粒

货号：kl-zl-0647-01
培养条件：37℃，有氧，LB

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0647-01	pCDNA3.1(+)/Myc-His B质粒	NA		立即咨询	+ -

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pCDNA3.1(+)/Myc-His B质粒货号：kl-zl-0647 规格：20ul

质粒描述：

启动子： CMV

复制子： pUC ori，F1 ori

终止子： BGH poly（A）signal

质粒分类：哺乳细胞载体;蛋白过表达质粒

质粒大小： 5497bp

质粒标签： 6X His, myc

原核抗性：氨苄青霉素Amp

筛选标记：新霉素Neo

克隆菌株： DH5α

培养条件： 37℃，有氧，LB

表达宿主：哺乳细胞

质粒简介：
All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
质粒图谱：

ORIGIN

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca

961 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaacaaaaac tcatctcaga

1021 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg

1081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc

1141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc

1201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa

1261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc

1321 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc

1381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct

1441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg

1501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga

1561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt

1621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg

1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc

1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg

1801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa

1861 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc

1921 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg

1981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt

2041 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga

2101 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt

2161 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg

2221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag

2281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt

2341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta

2401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg

2461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt

2521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat

2581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg

2641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca

2701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc

2761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc

2821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat

2881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc

2941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga

3001 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga

3061 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg

3121 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat

3181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt

3241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg

3301 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg

3361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc

3421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt

3481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg

3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt

3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc

3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa

3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa

3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc

3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc

3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag

3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga

4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc

4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac

4141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg

4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca

4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa

4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa

4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt

4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag

4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat

4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc

4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa

4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca

4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa

4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt

4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc

4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact

4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc

5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg

5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct

5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc

5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag

5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac

5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg

5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt

5461 tccgcgcaca tttccccgaa aagtgccacc tgacgtc

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