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pRSFDuet-1质粒

货号：kl-zl-0631-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0631-01	pRSFDuet-1质粒	NA		立即咨询	+ -

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pRSFDuet-1质粒货号：kl-zl-0631 规格：20ul

启动子：	T7/Lac
复制子：	RSF ori
终止子：	T7 terminator
质粒分类：	大肠系列质粒；大肠其它质粒；大肠双框质粒
质粒大小：	3829bp
质粒标签：	N-6×His，C-S
原核抗性：	卡那青霉素Kan（50μg/ml）
克隆菌株：	DH5α等大肠杆菌
培养条件：	37℃，有氧，LB
表达宿主：	BL21(DE3)等大肠杆菌
诱导方式：	IPTG或乳糖及其类似物
5'测序引物：	ACYCDuetUP1-F(GGATCTCGACGCTCTCCCT) DuetUP2-F( TTGTACACGGCCGCATAATC)
3'测序引物：	DuetDOWN1-R( GATTATGCGGCCGTGTACAA) T7-ter-R( TGCTAGTTATTGCTCAGCGG)
备注：	同时表达两个外源蛋白

质粒简介：
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pCDFDuet™-1 (Cat. Np. 71340-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
质粒图谱：

序列：

ORIGIN

1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag

121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag

181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt

241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata

301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt

361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg

421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg

481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg

541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt

601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt

661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat

721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt

781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa

841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg

901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa

961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca

1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc

1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca

1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt

1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt

1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat

1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc

1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt

1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat

1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt

1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt

1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac

1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt

1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa

1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc

1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc

1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg

1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt

2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg

2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac

2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag

2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc

2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta

2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca

2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc

2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg

2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga

2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt

2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc

2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg

2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt

2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact

2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc

2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc

3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga

3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa

3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg

3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag

3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg

3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc

3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc

3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga

3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg

3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa

3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct

3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg

3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg

3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata

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