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pCDNA3.1(-)质粒

货号：kl-zl-0357-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0357-01	pCDNA3.1(-)质粒	NA		立即咨询	+ -

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pCDNA3.1(-)质粒货号：kl-zl-0357 规格：20ul

质粒类型：	哺乳动物表达载体
启动子：	CMV
表达水平：	高
克隆方法：	多克隆位点，限制性内切酶
载体大小：	5427 bp
5' 测序引物：	pcDNA3.1-F：CTAGAGAACCCACTGCTTAC
3' 测序引物：	pcDNA3.1-R (BGH-R)：TAGAAGGCACAGTCGAGG
载体抗性：	Ampicillin (氨苄青霉素)
筛选标记：	Neomycin/G418
备注：	Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

质粒简介：
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements： • Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells • Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning • Neomycin resistance gene for selection of stable cell lines • Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
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