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pACYC177质粒

货号：kl-zl-0176-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0176-01	pACYC177质粒	NA		立即咨询	+ -

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pACYC177质粒货号：kl-zl-0176

复制子：	p15A ori
质粒分类：	大肠杆菌载体；克隆质粒
质粒大小：	3941bp
原核抗性：	氨苄青霉素Amp；卡那霉素Kan
克隆菌株：	DH5α
培养条件：	37℃，有氧，LB
5'测序引物：	根据序列设计
3'测序引物：	根据序列设计
备注：	丰富的酶切位点

质粒简介：
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
质粒图谱：

promoter 3594..3698

/gene="bla"

/note="AmpR promoter"

CDS 3699..618

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

ORIGIN

1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt

61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc

121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga

181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat

241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct

301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc

361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg

421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc

481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg

541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca

601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta

661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc

721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct

781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg

841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt

901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc

961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct

1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg

1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg

1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa

1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg

1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc

1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc

1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga

1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg

1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg

1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg

1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac

1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca

1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc

1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata

1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg

1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg

1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa

2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta

2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc

2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg

2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata

2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc

2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt

2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga

2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct

2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag

2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt

2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata

2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt

2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac

2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc

2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg

2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc

3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt

3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag

3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc

3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac

3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg

3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag

3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt

3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg

3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg

3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa

3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac

3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg

3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc

3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg

3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga

3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t

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