培养条件：
37℃
二,质粒简介：
pUC57-IGF1(145-354)(NM_001111283.2) 是一个人源cDNA基因克隆质粒。
三,质粒序列及图谱：
序列：
promoter complement(2760..2864)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatcggatcc cagtggatcc gatgatgatg ataaaggacc ggagacgctc
481 tgcggggctg agctggtgga tgctcttcag ttcgtgtgtg gagacagggg cttttatttc
541 aacaagccca cagggtatgg ctccagcagt cggagggcgc ctcagacagg catcgtggat
601 gagtgctgct tccggagctg tgatctaagg aggctggaga tgtattgcgc acccctcaag
661 cctgccaagt cagcttaatg agaattcagt gggcccgtcg actgcagagg cctgcatgca
721 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
841 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
901 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
1021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
1081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
1141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
1201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
1261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
1321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
1381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
1441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
1501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
1561 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
1621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
1681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
1741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
1801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
1861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
1921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
1981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
2041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
2101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
2161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
2221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
2281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
2341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
2401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
2461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
2521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
2581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
2641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
2701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
2761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
2821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
2881 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
2941 atcacgaggc cctttcgtc
