pCL-Eco质粒

pCL-Eco质粒 货号：kl-zl-0981 规格：20ul

启动子：	CMV
复制子：	pUC ori，SV40 ori
终止子：	SV40 poly(A) signal
质粒分类：	病毒系列，逆转录病毒包装载体图
质粒大小：	12341bp
质粒标签：	gag-pol
原核抗性：	Amp
克隆菌株：	Stbl3
培养条件：	37℃，有氧 LB
表达宿主：	哺乳细胞
诱导方式：	无须诱导，瞬时表达
5'测序引物：	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物：	根据序列设计引物

质粒简介：
The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.
质粒图谱：

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