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质粒测序通用引物列表如下

时间:2019-06-16 20:50    点击量:

质粒测序通用引物列表如下

引物名称 序列(5'→3') 描述
3'AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer
5'AOX1 GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer
Polyhedrin forward AAATGATAACCATCTCGC Polyhedrin promoter, forward primer
pGP704-R AACAAGCCAGGGATGTAACG R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer
SeqL-B (ATTL2) AACATCAGAGATTTTGAGACAC  
BAC1 AACCATCTCGCAAATAAATA  
PIRES3-EGFP-R AACGCACACCGGCCTTATTC  
Ubx-F AACTCGTACTTTGAACAGGC Drosophila Ultrabithorax gene, forward primer
PBV220F AAGAAGGGCAGCATTCAAAG  
pbd-gal4-r AAGAGTTACTCAAGAACAAGAA  
pMRB101-F AAGATGCAGGCAGCTGAGTT HCMV major immediate-early protein (IE), forward primer
PB42AD-R AAGCCGACAACCTTGATTGGAG  
T7EEV AAGGCTAGAGTACTTAATACG  
Gal4-AD AATACCACTACAATGGAT 3' end of Gal4 activation domain, forward primer
GAL4-ADfor AATACCACTACAATGGATGATG 3' end of Gal4 activation domain, forward primer
pBRforEco AATAGGCGTATCACGAGGC In pBR322, upsteam of EcoRI site, forward primer
GAL1 AATATACCTCTATACTTTAACGTC S. cerevisiae GAL1 promoter, forward primer
NOSR AATCATCGCAAGACCGGC  
GW-5' AATCTCGCCGGATCCTAACT 5' end of Gateway cassette, reverse primer
AC5 ACACAAAGCCGCTCCATCAG Drosophila Actin 5C promoter, forward primer
T7(17base) ACATCCACTTTGCCTTTCTC  
RCAS-F ACATGGGTGGTGGTATAGCGCTTGCG 3' of Rous sarcoma virus (RSV) env gene, forward primer
GST-Tag ACCCAATGTGCCTGGATGCG  
pBABE 3' ACCCTAACTGACACACATTCC SV40 enhancer, 3' of MCS in pBABE vectors, reverse primer
V5 Reverse ACCGAGGAGAGGGTTAGGGAT V5 epitope, reverse primer
pLNCX-R ACCTACAGGTGGGGTCTTTCATTCCC  
BAC2 ACGCACAGAATCTAGCGCTT  
pLTet-F ACTGAGCACATCAGCAGGAC Lambda phage early leftward (pL) promoter, forward primer
pCEP-F AGAGCTCGTTTAGTGAACCG  
F27 AGAGTTTGATCCTGGCTCA  
3'AD AGATGGTGCACGATGCACAG  
L4440 AGCGAGTCAGTGAGCGAG 5' of MCS in L4440 vector, forward primer
M13 Reverse  (-48) AGCGGATAACAATTTCACAC  
M13/pUC Reverse AGCGGATAACAATTTCACACAGG In lacZ gene
M13R(-48) AGCGGATAACAATTTCACACAGGA In lacZ gene
LNCX AGCTCGTTTAGTGAACCGTCAGATC Human CMV promoter, forward primer
pLNCX-F AGCTCGTTTAGTGAACCGTCAGATCG  
DsRed1-C AGCTGGACATCACCTCCCACAACG 3' end of DsRed1, forward primer
2.0rev primer AGGCGATTAAGTTGGGTA  
CMV-Reverse AGTAGGAAAGTCCCGTAAGG  
Luc-F AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer
N-CMV-30 ATAACCCCGCCCCGTTG  
Amp-R ATAATACCGCGCCACATAGC 5' end of ampicillin resistance gene, reverse primer
MMLV-F ATCAGTTCGCTTCTCGCTTC Moloney murine leukemia virus LTR (MoMuLV), forward primer
pBAD Forward ATGCCATAGCATTTTTATCC For vectors with E. coli araBAD promoter, forward primer
pET-Upstream ATGCGTCCGGCGTAGA  
U6 ATGGACTATCATATGCTTACCGTA  
pENTR-R ATGGCTCATAACACCCCTTG 3' of attL2 in pENTR vector, reverse primer
T3 ATTAACCCTCACTAAAGGGA T3 promoter, forward primer
pCMV5R ATTATAGAGGACACCTAGTC  
pDsRed-C-3' ATTCATTTTATGTTTCAGGTTCA  
SFFV-F ATTGATTGACTGCCCACCTC Spleen focus forming virus 5' LTR, forward primer
SP6 ATTTAGGTGACACTATAG SP6 promoter, forward primer
S1 CAACGTGAAAAAATTATTATTCGC  
TRC-F CAAGGCTGTTAGAGAGATAATTGGA Human U6 promoter, forward primer
T3 CAATTAACCCTCACTAAAGG  
AUG1 Forward CAATTTACATCTTTATTTATTAACG For Pichia vectors with AUG1 promoter, forward primer
SV40-spliceR CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer
pIRES.R CACTGCATTCTAGTTGTGGT  
mU6-F CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer
MSCV-rev CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer
M13 Reverse CAGGAAACAGCTATGAC In lacZ gene
M13R CAGGAAACAGCTATGACC  
mU6 CAGTGCAGGGGAAAGAATAGTAGAC  
WPRE-R CATAGCGTAAAAGGAGCAACA 5' end of WPRE, reverse primer
MT Forward CATCTCAGTGCAACTAAA Drosophila metallothionein promoter, forward primer
EGFP-C CATGGTCCTGCTGGAGTTCGTG 3' end of EGFP, forward primer
mPGK-F CATTCTGCACGCTTCAAAAG Mouse PGK promoter, forward primer
pTRE 3' CCACACCTCCCCCTGAAC  
PB42AD-F CCAGCCTCTTGCTGAGTGGAGATG  
hGH-PA-R CCAGCTTGGTTCCCAATAGA Human growth hormone terminator, reverse primer
Rluc-F CCAGGATTCTTTTCCAATGC 3' end of Renilla luciferase, forward primer
pTarget.F CCAGGATTTTCCCAGTCAC  
pCAMBIA1301-5' CCAGGCTTTACACTTTATGC  
GFP-R CCATCTAATTCAACAAGAATTGGGACAAC 5' end of GFP, reverse primer
M13/pUC Forward CCCAGTCACGACGTTGTAAAACG In lacZ gene
mCherry-F CCCCGTAATGCAGAAGAAGA 3' end of mCherry, forward primer
pQE promoter CCCGAAAAGTGCCACCTG 5' of MCS in pQE vectors, forward primer
Intein Forward CCCGCCGCTGCTTTTGCACGTGAG  
M13(-96) CCCTCATAGTTAGCGTAACG  
MSCV|pLXSN 5' CCCTTGAACCTCCTCGTTCGACC Murine stem cell virus, forward primer
pGEX 3' CCGGGAGCTGCATGTGTCAGAGG 3' of MCS in pGEX vectors, reverse primer
IRES-R CCTCACATTGCCAAAAGACG 5' end of IRES, reverse primer
SV40-pArev CCTCTACAAATGTGGTATGG  
LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer
S.tag CGAACGCCAGCACATGGACA  
CMV Forward CGCAAATGGGCGGTAGGCGTG Human CMV immediate early promoter, forward primer
OpIE2 Forward CGCAACGATCTGGTAAACAC OpIE2 promoter, forward primer
M13F(-47) CGCCAGGGTTTTCCCAGTCACGAC  
pTRE 5' CGCCTGGAGACGCCATCC  
pBluescript SK CGCTCTAGAACTAGTGGATC  
pRS-marker CGGCATCAGAGCAGATTGTA To sequence yeast selectable marker in pRS vectors
GPDpro-F CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer
pLEXA-F CGTCAGCAGAGCTTCACCAT  
LexA CGTCAGCAGAGCTTCACCATTG 3' end of LexA DNA binding domain, forward primer
EGFP-Nrev CGTCGCCGTCCAGCTC  
EGFP-N CGTCGCCGTCCAGCTCGACCA  
EGFP-N CGTCGCCGTCCAGCTCGACCAG 5' end of EGFP, reverse primer
PinPoint  primer CGTGACGCGGTGCAGGGCG  
Neo-F CGTTGGCTACCCGTGATATT 3' end of neomycin resistance gene, forward primer
pENTR-F CTACAAACTCTTCCTGTTAGTTAG 5' of attL1 in pENTR vector, forward primer
PCDNA3.1F CTAGAGAACCCACTGCTTAC  
RVprimer3 CTAGCAAAATAGGCTGTCCC 5' of MCS in pGL3 vector, forward primer
35S promoter CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer
5'TriplEx2 CTCCGAGATCTGGACGAGC  
Ecdysone Forward CTCTGAATACTTTCAACAAGTTAC Drosophila heat shock promoter, forward primer
pFastBac-3' CTGATTATGATCCTCTAGTACTTC  
PBV220R CTGCGTTCTGATTTAATCTG  
Bglob-intron-F CTGGTCATCATCCTGCCTTT Rabbit beta-globin intron, forward primer
tdTomato-Fwd CTGTTCCTGTACGGCATGG 3' end of tdTomato, forward primer
pIRES-F CTTACTGACATCCACTTTGC  
Pry1 CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer
TKpolyAreverse CTTCCGTGTTTCAGTTAGC  
pLXSN-F CTTGAACCTCCTCGTTCGAC  
pBRforBam CTTGGAGCCACTATCGAC In pBR322 tet region, upstream of BamHI, forward primer
pBABE 5' CTTTATCCAGCCCTCAC Psi packaging signal, 5' of MCS in pBABE vectors, forward  primer
GLP2 CTTTATGTTTTTGGCGTCTTCCA  
SV40pA-R GAAATTTGTGATGCTATTGC SV40 polyA, reverse primer
S.tag GAACGCCAGCACATGGAC  
AUG1 Reverse GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter, reverse primer
LacZ-R GACAGTATCGGCCTCAGGAA 5' end of LacZ, reverse primer
RVP4 GACGATAGTCATGCCCCGCG  
pbi-121(35s) GACGCACAATCCCACTATCC  
pMX-S1811 GACGGCATCGCAGCTTGGATACAC MMLV sequence, 5' of MCS in pMXs vector, forward primer
HU6 GACTATCATATGCTTACCGT  
XBG-R GACTCCATTCGGGTGTTC Xenopus beta-globin 3'UTR, reverse primer
Tac promoter GAGCGGATAACAATTTCACACAGG Tac promoter, forward primer
HAT GAGGAGCACGCTCATGCCCAC Histidine affinity tag, forward primer
pTrcHis Forward GAGGTATATATTAATGTATCG 5' of MCS in pTrcHis vector, forward primer
Gal4 N-term GAGTAGTAACAAAGGTCAA 3' end of Gal4 DNA binding domain, forward primer
pHybLex Reverse GAGTCACTTTAAAATTTGTATACAC ADH terminator, reverse primer
3.0rev GAGTTAGCTCACTCATTAGGC  
NOS GATAATCATCGCAAGACCGG  
pCMVF GATCCGGTACTAGAGGAACTGAAAAAC  
DuetUP1 GATCTCGACGCTCTCCCT  
MBP-F GATGAAGCCCTGAAAGACGCGCAG 3' end of maltose binding protein, forward primer
pYESTrp  Forward GATGTTAACGATACCAGCC  
DuetDOWN1 GATTATGCGGCCGTGTACAA  
pBAD Reverse|pTrcHis Reverse GATTTAATCTGTATCAGG For vectors with E. coli araBAD promoter, reverse primer
CRE-R GCAAACGGACAGAAGCATTT 5' end of Cre recombinase, reverse primer
Puro-F GCAACCTCCCTTCTACGAGC 3' end of puromycin resistance gene, forward primer
pCAG-F GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids, forward primer
pCasper-hs GCAACTACTGAAATCTGCCAAG Drosophila Hsp70 promoter, forward primer
CAT-R GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer
Nmt1-F GCAATGTGCAGCGAAACTAA S. pombe nmt1 promoter, forward primer
T3 GCAATTAACCCTCACTAAAGG T3 promoter, forward primer
pFlag-CMV-R GCACTGGAGTGGCAACTT  
Myc GCATCAATGCAGAAGCTGATCTCA Myc tag, forward primer
GW-3' GCATGATGACCACCGATATG 3' end of Gateway cassette, forward primer
Neo-R GCCCAGTCATAGCCGAATAG 5' end of neomycin resistance gene, reverse primer
SV40-Promoter GCCCCTAACTCCGCCCATCC  
pbd-gal4-f GCCTCTAACATTGAGACAGC  
SeqL-A (ATTL1) GCGAGAGTAGGGAACTGC  
pCAMBIA1301-3' GCGATTAAGTTGGGTAACGC  
CYC1|pYESTrp Reverse GCGTGAATGTAAGCGTGAC CYC1 transcription termination signal, reverse primer
NOS-F GCGTTCAAAAGTCGCCTAAG Nopaline synthase promoter, forward primer
pAd-CMV GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector
T7 Terminal GCTAGTTATTGCTCAGCGG T7 terminator, reverse primer
pREP Forward GCTCGATACAATAAACGCC Rous sarcoma virus (RSV) promoter, forward primer
MT1-F GCTGTCCTCTAAGCGTCACC Mouse metallothionein 1 promoter, forward primer
pBMN 5' GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector
M13 Reverse  (-27) GGAAACAGCTATGACCATG  
SV40-F GGAGGCTTTTTTGGAGGC  
3'AOX GGCAAATGGCATTCTGACAT  
PCDNA3.0-R2 GGCAACTAGAAGGCACAGTC  
LacI-R GGCATACTCTGCGACATCGT 5' end of LacI, reverse primer
Tet-R GGCGAGTTTACGGGTTGTTA 5' end of tetracycline resistance gene, reverse primer
pTarget.R GGCTTTACACTTTATGCTTC  
pBR322ori-F GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer
U6 Primer GGGCAGGAAGAGGGCCTAT  
pGEX 5' GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer
Tn7-end GGGGTGGAAATGGAGTTTTT Bacterial transposon Tn7
pCasper-F GGGTTTTATTAACTTACAT 5' end of Drosophila mini-white gene, reverse primer
pFlag-CMV-F GGTAGGCGTGTACGGTGG  
GFP-F GGTCCTTCTTGAGTTTGTAAC 3' end of GFP, forward primer
MalE Primer GGTCGTCAGACTGTCGATGAAGCC  
pSHUTTLE-CMV-F GGTCTATATAAGCAGAGGTG  
pBRrevBam GGTGATGTCGGCGATATAGG In pBR322 tet region, downstream of BamHI, reverse primer
Gal10pro-F GGTGGTAATGCCATGTAATATG S. cerevisiae GAL10 promoter, forward primer
1492R GGTTACCTTGTTACGACTT  
M13 Forward  (-20) GTAAAACGACGGCCAGTG  
S6 GTAAATGAATTTTCTGTAGTAGG  
pPc86-3' GTAAATTTCTGGCAAGGTAGAC  
DsRed1-N GTACTGGAACTGGGGGGACAG 5' end of DsRed1, reverse primer
PIRES2-EGFP-F GTAGGCGTGTACGGTGGGAG  
Polyhedrin reverse GTCCAAGTTTCCCTG For baculovirus vector with polyhedrin promoter, reverse  primer
pFastBac-5' GTCCGAAACCATGTCGTAC  
EXFP-R GTCTTGTAGTTGCCGTCGTC For distinguishing EGFP vs ECFP vs EYFP, reverse primer
pACT2-R GTGCACGATGCACAGTTGAA  
F1ori-F GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer
pSHUTTLE-CMV-R GTGGTATGGCTGATTATGATCAG  
EBV Reverse GTGGTTTGTCCAAACTCATC SV40 polyA terminator, reverse primer
CMV-R GTTCACGGTGCCCTCC  
pQE30-R GTTCTGAGGTCATTACTGG  
pcDL-F GTTGCCTTTACTTCTAGGCCT 5' of EcoRI site in pcDL vector, forward primer
pLXSN-R GTTGCTGACTAATTGAGATG  
M13 (-40) GTTTTCCCAGTCACGAC In lacZ gene
pLEXA-R TAAAACCTAAGAGTCACTTT  
3'BD TAAGAGTCACTTTAAAATTTGTA  
T7 TAATACGACTCACTATAGGG T7 promoter, forward primer
GAL4 AD TACCACTACAATGGATG  
HA-F TACCCATACGACGTCCCAGA HA tag, forward primer
SP6 TACGATTTAGGTGACACTATAG  
α-Factor TACTATTGCCAGCATTGCTGC  
BGH Reverse|pcDNA3.1R TAGAAGGCACAGTCGAGG Bovine growth hormone terminator, reverse primer
pPc86F TATAACGCGTTTGGAATCACT  
Xpress Forward TATGGCTAGCATGACTGGT Xpress epitope, forward primer
PEGFP-C TATGGCTGATTATGATCAGT  
SV40pro-F TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer
EF-1a Forward TCAAGCCTCAGACAGTGGTTC Human elongation factor-1a promoter, forward primer
GAL4 BD TCATCGGAAGAGAGTAG  
GAL4-BDfor TCATCGGAAGAGAGTAGTAA  
pCMV-R TCCAAACTCATCAATGTATC  
pDsRED-express-C1-F TCCCACAACGAGGACTACAC  
hrGFP-R TCCCCGAGTACCACTTCATC hrGFP (humanized Renilla GFP), forward primer
ITS1 TCCGTAGGTGAACCTGCGG  
ITS4 TCCTCCGCTTATTGATATGC  
pZIP TCCTTTCCAGCGAGGTTCTA Murine leukemia virus (MuLV), reverse primer
pBluescriptKS TCGAGGTCGACGGTATC For pBluescript vector
H1 TCGCTATGTGTTCTGGGAAA Human H1 promoter, forward primer
pYes2.R TCGGTTAGAGCGGATGTG  
pCMV-F TCTAAAAGCTGCGGAATTGT  
pBluescriptSK TCTAGAACTAGTGGATC For pBluescript vector
HA-R TCTGGGACGTCGTATGGGTA HA tag, reverse primer
tdTomato-Rev TCTTTGATGACGGCCATGT 5' end of tdTomato, reverse primer
pDSRED-N1-R TGAAGCGCATGAACTCCTTG  
hUBCpro-F TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter, forward primer
pACYC-F TGAAGTCAGCCCCATACGAT p15A origin, forward primer
pQE30-F TGAGCGGATAACAATTTCAC  
P5'|pMAL-C2X-F TGCGTACTGCGGTGATCAAC  
T7TER TGCTAGTTATTGCTCAGCGG  
IRES-F TGGCTCTCCTCAAGCGTATT 3' end of IRES, forward primer
pEGFP-N-5’ TGGGAGGTCTATATAAGCAGAG  
M13 (-21) Forward TGTAAAACGACGGCCAGT In lacZ gene
GLP1 TGTATCTTATGGTACTGTAACTG  
C-CMV-24 TTAGGACAAGGCTGGTGG  
PCMV-5F TTCCAAAATGTCGTAATAAC  
pMT2-F TTGCCTTTCTCTCCACAGGT 3' end of synthetic intron, forward primer
pTRC99C-F TTGCGCCGACATCATAAC  
mCherry-R TTGGTCACCTTCAGCTTGG 5' end of mCherry, reverse primer
DuetUP2 TTGTACACGGCCGCATAATC  
TK-pA-R TTGTCTCCTTCCGTGTTTCA Thymidine kinase polyA, reverse primer
XEF1a TTTCGCCCTAACTTCGTGAT Xenopus EF1 alpha enhancer/promoter, forward primer
Bglob-intron-R TTTGCCCCCTCCATATAACA Rabbit beta-globin intron, reverse primer
P12584 TTTTCAGTATCTACGAT  
Bglob-pA-R TTTTGGCAGAGGGAAAAAGA Rabbit beta-globin polyA region, reverse primer
 

 
 

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