别名： J02151.1
原核抗性： 氨苄青霉素
筛选标记： 遗传霉素G418
克隆菌株： 大肠杆菌DH5α
培养条件： 37℃
pCDNA3.1-H1N1-Polymerase 是一个甲型流感病毒聚合酶基因哺乳表达质粒。Influenza A virus (A/Puerto Rico/8/34(H1N1)) polymerase 1 gene, complete cds；GenBank： J02151.1.
序列：
CDS complement(6656..7516)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7517..7621)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgga
901 tgtcaatccg accttacttt tcttaaaagt gccagcacaa aatgctataa gcacaacttt
961 cccttatacc ggagaccctc cttacagcca tgggacagga acaggataca ccatggatac
1021 tgtcaacagg acacatcagt actcagaaaa ggcaagatgg acaacaaaca ccgaaactgg
1081 agcaccgcaa ctcaacccga ttgatgggcc actgccagaa gacaatgaac caagtggtta
1141 tgcccaaaca gattgtgtat tggaagcaat ggctttcctt gaggaatccc atcctggtat
1201 ttttgaaaac tcgtgtattg aaacgatgga ggttgttcag caaacacgag tagacaagct
1261 gacacaaggc cgacagacct atgactggac tttaaataga aaccagcctg ctgcaacagc
1321 attggccaac acaatagaag tgttcagatc aaatggcctc acggccaatg agtctggaag
1381 gctcatagac ttccttaagg atgtaatgga gtcaatgaaa aaagaagaaa tggggatcac
1441 aactcatttt cagagaaaga gacgggtgag agacaatatg actaagaaaa tgataacaca
1501 gagaacaata ggtaaaagga aacagagatt gaacaaaagg agttatctaa ttagagcatt
1561 gaccctgaac acaatgacca aagatgctga gagagggaag ctaaaacgga gagcaattgc
1621 aaccccaggg atgcaaataa gggggtttgt atactttgtt gagacactgg caaggagtat
1681 atgtgagaaa cttgaacaat cagggttgcc agttggaggc aatgagaaga aagcaaagtt
1741 ggcaaatgtt gtaaggaaga tgatgaccaa ttctcaggac accgaacttt ctttgaccat
1801 cactggagat aacaccaaat ggaacgaaaa tcagaatcct cggatgtttt tggccatgat
1861 cacatatatg accagaaatc agcccgaatg gttcagaaat gttctaagta ttgctccaat
1921 aatgttctca aacaaaatgg cgagactggg aaaagggtat atgtttgaga gcaagagtat
1981 gaaacttaga actcaaatac ctgcagaaat gctagcaagc attgatttga aatatttcaa
2041 tgattcaaca agaaagaaga ttgaaaaaat ccgaccgctc ttaatagagg ggactgcatc
2101 attgagccct ggaatgatga tgggcatgtt caatatgtta agcactgtat taggcgtctc
2161 catcctgaat cttggacaaa agagatacac caagactact tactggtggg atggtcttca
2221 atcctctgac gattttgctc tgattgtgaa tgcacccaat catgaaggga ttcaagccgg
2281 agtcgacagg ttttatcgaa cctgtaagct acatggaatc aatatgagca agaaaaagtc
2341 ttacataaac agaacaggta catttgaatt cacaagtttt ttctatcgtt atgggtttgt
2401 tgccaatttc agcatggagc ttcccagttt tggtgtgtct gggagcaacg agtcagcgga
2461 catgagtatt ggagttactg tcatcaaaaa caatatgata aacaatgatc ttggtccagc
2521 aacagctcaa atggcccttc agttgttcat caaagattac aggtacacgt accgatgcca
2581 tagaggtgac acacaaatac aaacccgaag atcatttgaa ataaagaaac tgtgggagca
2641 aacccgttcc aaagctggac tgctggtctc cgacggaggc ccaaatttat acaacattag
2701 aaatctccac attcctgaag tctgcctaaa atgggaattg atggatgagg attaccaggg
2761 gcgtttatgc aacccactga acccatttgt cagccataaa gaaattgaat caatgaacaa
2821 tgcagtgatg atgccagcac atggtccagc caaaaacatg gagtatgatg ctgttgcaac
2881 aacacactcc tggatcccca aaagaaatcg atccatcttg aatacaagtc aaagaggagt
2941 acttgaagat gaacaaatgt accaaaggtg ctgcaattta tttgaaaaat tcttccccag
3001 cagttcatac agaagaccag tcgggatatc cagtatggtg gaggctatgg tttccagagc
3061 ccgaattgat gcacggattg atttcgaatc tggaaggata aagaaagaag agttcactga
3121 gatcatgaag atctgttcca ccattgaaga gctcagacgg caaaaaggag gcggcggttc
3181 ccatcaccac catcaccacc atcattgact cgagtctaga gggcccgttt aaacccgctg
3241 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
3301 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
3361 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
3421 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
3481 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
3601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
4201 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
4321 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
4381 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
4441 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
4501 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
4561 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
4621 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
4681 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
4741 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
4801 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
4861 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
4921 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
4981 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
5041 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
5101 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
5161 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
5221 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
5281 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
5341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
5401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
5461 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
5521 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
5581 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
5641 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
5701 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5761 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5821 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
5881 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
5941 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
6001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
6061 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
6121 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
6181 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
6241 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
6301 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
6361 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6421 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
6481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
6541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
6601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
6661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
6721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
6781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
6841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
6901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
6961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
7021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
7081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
7141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
7201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
7261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
7321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
7381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
7441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
7501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
7561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
7621 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
7681 atggt
